Differential distribution and intracellular targeting of mRNAs corresponding to the three calmodulin genes in rat brain

A quantitative in situ hybridization study

Arpad Palfi, Sandor Vizi, K. Gulya

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

To investigate the pattern of expression of the three calmodulin (CAM) genes by in situ hybridization, gene-specific [35S]-cRNA probes complementary to the multiple CaM mRNAs were hybridized in rat brain sections and subsequently detected by quantitative film or high-resolution nuclear emulsion autoradiography. A widespread and differential area-specific distribution of the CaM mRNAs was detected. The expression patterns corresponding to the three CaM genes differed most considerably in the olfactory bulb, the cerebral and cerebellar cortices, the diagonal band, the suprachiasmatic and medial habenular nuclei, and the hippocampus. Moreover, the significantly higher CaM I and CaM III mRNA copy numbers than that of CaM II in the molecular layers of certain brain areas revealed a differential dendritic targeting of these mRNAs. The results indicate a differential pattern of distribution of the multiple CaM mRNAs at two levels of cellular organization in the brain: (a) region-specific expression and (b) specific intracellular targeting. A precise and gene-specific regulation of synthesis and distribution of CaM mRNAs therefore exists under physiological conditions in the rat brain.

Original languageEnglish
Pages (from-to)583-600
Number of pages18
JournalJournal of Histochemistry and Cytochemistry
Volume47
Issue number5
Publication statusPublished - May 1999

Fingerprint

Calmodulin
In Situ Hybridization
Messenger RNA
Brain
Genes
Habenula
Complementary RNA
Cerebellar Cortex
Olfactory Bulb
Emulsions
Autoradiography
Cerebral Cortex
Hippocampus

Keywords

  • Calmodulin mRNAs
  • Dendritic targeting
  • Gene expression
  • Image analysis
  • In situ hybridization
  • mRNA localization
  • Quantitative autoradiography
  • Rat brain

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

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abstract = "To investigate the pattern of expression of the three calmodulin (CAM) genes by in situ hybridization, gene-specific [35S]-cRNA probes complementary to the multiple CaM mRNAs were hybridized in rat brain sections and subsequently detected by quantitative film or high-resolution nuclear emulsion autoradiography. A widespread and differential area-specific distribution of the CaM mRNAs was detected. The expression patterns corresponding to the three CaM genes differed most considerably in the olfactory bulb, the cerebral and cerebellar cortices, the diagonal band, the suprachiasmatic and medial habenular nuclei, and the hippocampus. Moreover, the significantly higher CaM I and CaM III mRNA copy numbers than that of CaM II in the molecular layers of certain brain areas revealed a differential dendritic targeting of these mRNAs. The results indicate a differential pattern of distribution of the multiple CaM mRNAs at two levels of cellular organization in the brain: (a) region-specific expression and (b) specific intracellular targeting. A precise and gene-specific regulation of synthesis and distribution of CaM mRNAs therefore exists under physiological conditions in the rat brain.",
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T1 - Differential distribution and intracellular targeting of mRNAs corresponding to the three calmodulin genes in rat brain

T2 - A quantitative in situ hybridization study

AU - Palfi, Arpad

AU - Vizi, Sandor

AU - Gulya, K.

PY - 1999/5

Y1 - 1999/5

N2 - To investigate the pattern of expression of the three calmodulin (CAM) genes by in situ hybridization, gene-specific [35S]-cRNA probes complementary to the multiple CaM mRNAs were hybridized in rat brain sections and subsequently detected by quantitative film or high-resolution nuclear emulsion autoradiography. A widespread and differential area-specific distribution of the CaM mRNAs was detected. The expression patterns corresponding to the three CaM genes differed most considerably in the olfactory bulb, the cerebral and cerebellar cortices, the diagonal band, the suprachiasmatic and medial habenular nuclei, and the hippocampus. Moreover, the significantly higher CaM I and CaM III mRNA copy numbers than that of CaM II in the molecular layers of certain brain areas revealed a differential dendritic targeting of these mRNAs. The results indicate a differential pattern of distribution of the multiple CaM mRNAs at two levels of cellular organization in the brain: (a) region-specific expression and (b) specific intracellular targeting. A precise and gene-specific regulation of synthesis and distribution of CaM mRNAs therefore exists under physiological conditions in the rat brain.

AB - To investigate the pattern of expression of the three calmodulin (CAM) genes by in situ hybridization, gene-specific [35S]-cRNA probes complementary to the multiple CaM mRNAs were hybridized in rat brain sections and subsequently detected by quantitative film or high-resolution nuclear emulsion autoradiography. A widespread and differential area-specific distribution of the CaM mRNAs was detected. The expression patterns corresponding to the three CaM genes differed most considerably in the olfactory bulb, the cerebral and cerebellar cortices, the diagonal band, the suprachiasmatic and medial habenular nuclei, and the hippocampus. Moreover, the significantly higher CaM I and CaM III mRNA copy numbers than that of CaM II in the molecular layers of certain brain areas revealed a differential dendritic targeting of these mRNAs. The results indicate a differential pattern of distribution of the multiple CaM mRNAs at two levels of cellular organization in the brain: (a) region-specific expression and (b) specific intracellular targeting. A precise and gene-specific regulation of synthesis and distribution of CaM mRNAs therefore exists under physiological conditions in the rat brain.

KW - Calmodulin mRNAs

KW - Dendritic targeting

KW - Gene expression

KW - Image analysis

KW - In situ hybridization

KW - mRNA localization

KW - Quantitative autoradiography

KW - Rat brain

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