Differential detergent sensitivity of extracellular vesicle subpopulations

Xabier Osteikoetxea, Barbara Sódar, Andrea Németh, Katalin Szabó-Taylor, Krisztina Pálóczi, Krisztina V. Vukman, V. Tamási, Andrea Balogh, A. Kittel, E. Pállinger, E. Búzás

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Extracellular vesicles (including exosomes, microvesicles and apoptotic bodies) are currently attracting rapidly increasing attention from various fields of biology due to their ability to carry complex information and act as autocrine, paracrine and even endocrine intercellular messengers. In the present study we investigated the sensitivity of size-based subpopulations of extracellular vesicles to different concentrations of detergents including sodium dodecyl sulphate, Triton X-100, Tween 20 and deoxycholate. We determined the required detergent concentration that lysed each of the vesicle subpopulations secreted by Jurkat, THP-1, MiaPaCa and U937 human cell lines. We characterized the vesicles by tunable resistive pulse sensing, flow cytometry and transmission electron microscopy. Microvesicles and apoptotic bodies were found to be more sensitive to detergent lysis than exosomes. Furthermore, we found evidence that sodium dodecyl sulphate and Triton X-100 were more effective in vesicle lysis at low concentrations than deoxycholate or Tween 20. Taken together, our data suggest that a combination of differential detergent lysis with tunable resistive pulse sensing or flow cytometry may prove useful for simple and fast differentiation between exosomes and other extracellular vesicle subpopulations as well as between vesicular and non-vesicular structures.

Original languageEnglish
Pages (from-to)9775-9782
Number of pages8
JournalOrganic and Biomolecular Chemistry
Volume13
Issue number38
DOIs
Publication statusPublished - Aug 3 2015

Fingerprint

detergents
Detergents
Exosomes
Deoxycholic Acid
cytometry
Flow cytometry
Polysorbates
sensitivity
Octoxynol
sodium sulfates
Sodium Dodecyl Sulfate
Flow Cytometry
pulses
U937 Cells
biology
cultured cells
low concentrations
Transmission Electron Microscopy
Cells
Transmission electron microscopy

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Biochemistry

Cite this

Osteikoetxea, X., Sódar, B., Németh, A., Szabó-Taylor, K., Pálóczi, K., Vukman, K. V., ... Búzás, E. (2015). Differential detergent sensitivity of extracellular vesicle subpopulations. Organic and Biomolecular Chemistry, 13(38), 9775-9782. https://doi.org/10.1039/c5ob01451d

Differential detergent sensitivity of extracellular vesicle subpopulations. / Osteikoetxea, Xabier; Sódar, Barbara; Németh, Andrea; Szabó-Taylor, Katalin; Pálóczi, Krisztina; Vukman, Krisztina V.; Tamási, V.; Balogh, Andrea; Kittel, A.; Pállinger, E.; Búzás, E.

In: Organic and Biomolecular Chemistry, Vol. 13, No. 38, 03.08.2015, p. 9775-9782.

Research output: Contribution to journalArticle

Osteikoetxea, X, Sódar, B, Németh, A, Szabó-Taylor, K, Pálóczi, K, Vukman, KV, Tamási, V, Balogh, A, Kittel, A, Pállinger, E & Búzás, E 2015, 'Differential detergent sensitivity of extracellular vesicle subpopulations', Organic and Biomolecular Chemistry, vol. 13, no. 38, pp. 9775-9782. https://doi.org/10.1039/c5ob01451d
Osteikoetxea X, Sódar B, Németh A, Szabó-Taylor K, Pálóczi K, Vukman KV et al. Differential detergent sensitivity of extracellular vesicle subpopulations. Organic and Biomolecular Chemistry. 2015 Aug 3;13(38):9775-9782. https://doi.org/10.1039/c5ob01451d
Osteikoetxea, Xabier ; Sódar, Barbara ; Németh, Andrea ; Szabó-Taylor, Katalin ; Pálóczi, Krisztina ; Vukman, Krisztina V. ; Tamási, V. ; Balogh, Andrea ; Kittel, A. ; Pállinger, E. ; Búzás, E. / Differential detergent sensitivity of extracellular vesicle subpopulations. In: Organic and Biomolecular Chemistry. 2015 ; Vol. 13, No. 38. pp. 9775-9782.
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