Differential detection of N-heterocyclic compounds and their N-methylated derivatives by immunoanalysis

Hong M. Le, Gyöngyvér Hegedüs, A. Székács

Research output: Contribution to journalArticle

Abstract

Competitive enzyme-linked immunosorbent assay (ELISA) systems are proposed for the indirect monitoring of formaldehyde by the parallel detection of its N-methylated precursors and the corresponding demethylated compounds. As an example for such immunoanalytical differentiation between an N-heterocyclic compound and its N-methylated derivative, the quantitative detection of the systemic triazole fungicide, myclobutanil, is discussed. Antibodies recognizing the non-zwitterionic structure of 2-(4-chlorophenyl)-2-[(1,2,4-triazol-1-yl)-methyl]-hexanonitrile (myelobutanil) showed only minor binding to corresponding N-alkylated derivatives of myelobutanil. And vice versa, literature data indicate that antibodies raised against the pyridilium ionic structure of the herbicide paraquat, displayed only mediocre reactivity towards the corresponding dealkylated derivatives. Thus, both experimental and literature data suggest that immunoanalytical methods for differential detection of N-methylated heterocycles (potentially including formaldehyde precursors) and their non-methylated counterparts are possible to develop.

Original languageEnglish
Pages (from-to)455-462
Number of pages8
JournalActa Biologica Hungarica
Volume49
Issue number2-4
Publication statusPublished - 1998

Fingerprint

heterocyclic compounds
Heterocyclic Compounds
Formaldehyde
Derivatives
formaldehyde
Paraquat
antibody
Triazoles
Antibodies
Herbicides
triazole fungicides
myclobutanil
Fungicides
paraquat
Immunosorbents
antibodies
Enzyme-Linked Immunosorbent Assay
fungicide
herbicide
Assays

Keywords

  • Cross-reactivity
  • ELISA
  • Immunoassay
  • Myclobutanil
  • Paraquat

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Differential detection of N-heterocyclic compounds and their N-methylated derivatives by immunoanalysis. / Le, Hong M.; Hegedüs, Gyöngyvér; Székács, A.

In: Acta Biologica Hungarica, Vol. 49, No. 2-4, 1998, p. 455-462.

Research output: Contribution to journalArticle

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