Differential changes in presynaptic modulation of transmitter release during aging

G. Zsilla, T. Zelles, A. Mike, A. Kékes-Szabó, E. Milusheva, E. Vízi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The purpose of this study was to assess the functional role of presynaptic α2-autoreceptors in noradrenergic transmission in the hippocampus and dopamine-2 heteroreceptors in cholinergic transmission in the striatum in young, adult, and senescent rats. Male and female Wistar rats (4, 12, and 24 months old) were used and the release of radioactivity from striatal and hippocampal slices that had been loaded either with [3H]choline or with [3H]norepinephrine was measured at rest and in response to field stimulation (2 Hz, 360 shocks). The release was challenged by sulpiride, a selective dopamine-2 receptor antagonist, and CH-38083, a selective α2-adrenoceptor antagonist. The dissociation constant and the number of α2-adrenoceptors was also determined by binding studies using [3H]yohimbine as ligand in crude membrane preparations of frontal cortex. There were an age-related changes in α2-adrenoceptor-mediated negative feedback modulation of norepinephrine release and in the density and dissociation constant of α2-adrenoceptors. They were reduced in senescent rats. In contrast the presynaptic modulation of striatal cholinergic transmission by dopamine-2 receptors was not altered during aging, but the storage capacity of and the release of acetylcholine from cholinergic interneurons was significantly lower.

Original languageEnglish
Pages (from-to)107-115
Number of pages9
JournalInternational Journal of Developmental Neuroscience
Volume12
Issue number2
DOIs
Publication statusPublished - 1994

Fingerprint

Adrenergic Receptors
Cholinergic Agents
Corpus Striatum
Norepinephrine
Autoreceptors
Sulpiride
Yohimbine
Dopamine Antagonists
Dopamine Receptors
Frontal Lobe
Interneurons
Choline
Radioactivity
Acetylcholine
Wistar Rats
Young Adult
Shock
Hippocampus
Dopamine
Ligands

Keywords

  • acetylcholine release
  • ageing
  • norepinephrine release
  • presynaptic modulation
  • [H]yohimbine binding

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Differential changes in presynaptic modulation of transmitter release during aging. / Zsilla, G.; Zelles, T.; Mike, A.; Kékes-Szabó, A.; Milusheva, E.; Vízi, E.

In: International Journal of Developmental Neuroscience, Vol. 12, No. 2, 1994, p. 107-115.

Research output: Contribution to journalArticle

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