Differential adhesion of MBP specific CD4+ T cell hybridoma clones to cerebromicrovascular and peripheral endothelium

J. Joseph, B. Kálmán, K. Siebert, F. D. Lublin, R. L. Knobler

Research output: Contribution to journalArticle

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Abstract

Experimental allergic encephalomyelitis (EAE) is an organ specific disease, mediated by T cells trafficking into the central nervous system (CNS) (1) and has served as a model system for the human demyelinating disease, multiple sclerosis (MS) (2). The goal of the present study was to determine if CNS-specific lymphocyte-endothelial interactions exist for CNS antigen specific T cells. The adhesion of MBP specific CD4+ T cell hybridoma clones to untreated and cytokine activated CNS and peripheral (hepatic) endothelium was compared. A non-CNS antigen, chicken ovalbumin specific, T cell hybridoma was used as a control. Increased binding of the MBP specific CD4+ T cell hybridoma to CNS endothelium was observed when compared to peripheral endothelium. Increased, but persistently differential binding to CNS endothelium was also observed in the cytokine (TNF, γ-IFN) treated groups. In contrast, a non-CNS antigen specific control cell line did not demonstrate selective binding to brain vs. peripheral endothelium. These results demonstrate organ specific adhesive interactions between MBP specific T cells and CNS endothelium.

Original languageEnglish
Pages (from-to)278-284
Number of pages7
JournalRegional Immunology
Volume6
Issue number4
Publication statusPublished - 1994

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Hybridomas
Endothelium
Central Nervous System
Clone Cells
T-Lymphocytes
Antigens
Nervous System
Cytokines
Autoimmune Experimental Encephalomyelitis
Ovalbumin
Demyelinating Diseases
Adhesives
Multiple Sclerosis
Chickens
Lymphocytes
Cell Line
Liver
Brain

ASJC Scopus subject areas

  • Immunology

Cite this

Differential adhesion of MBP specific CD4+ T cell hybridoma clones to cerebromicrovascular and peripheral endothelium. / Joseph, J.; Kálmán, B.; Siebert, K.; Lublin, F. D.; Knobler, R. L.

In: Regional Immunology, Vol. 6, No. 4, 1994, p. 278-284.

Research output: Contribution to journalArticle

Joseph, J. ; Kálmán, B. ; Siebert, K. ; Lublin, F. D. ; Knobler, R. L. / Differential adhesion of MBP specific CD4+ T cell hybridoma clones to cerebromicrovascular and peripheral endothelium. In: Regional Immunology. 1994 ; Vol. 6, No. 4. pp. 278-284.
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