Different types of false positive anti-HIV reactions in patients on haemodialysis

E. Újhelyi, G. Füst, Gábor Illei, Éva Gyodi, Károly Nagy, F. Tóth, Béla Büki, Manfred P. Dierich, Vlastimil Mayer, Gyorgy Gal, János Mako, S. Hollán

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Abstract

Serum samples of 589 haemodialysis patients were sceened for HIV antibody by ELISA methods. Of these, 36 samples were found to be repeatedly reactive. None of the 36, however, could be confirmed by competitive enzyme immunoassays and Western blot; therefore, they were considered to be false positive. The sera could be divide in two groups. The sera of Group 1 were designated as the usual type of false positivity, caused most probably by anti-lymphocyte antibodies. In 19 sera, however, a special type of false positivity was found. These sera reacted strongly with the plate coated with the supernatants of HIV-infected cells but not with those of uninfected H9 cells. Three and two sera showed, respectively, positive immunofluorescence reaction with the HIV-infected, but not with the uninfected, H9 and CEM cells. Reactivity to HIV-infected H9 cells could be adsorbed from a part of these samples with lesser amounts of HIV-infected than uninfected H9 cells. This special type of false positivity was observed frequently (7/65) in patients who rejected a kidney graft. These findings suggest that this type of anti-HIV false positivity is due to.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalImmunology Letters
Volume22
Issue number1
DOIs
Publication statusPublished - 1989

Fingerprint

Renal Dialysis
HIV
Serum
HIV Seropositivity
HIV Antibodies
Immunoenzyme Techniques
Fluorescent Antibody Technique
Anti-Idiotypic Antibodies
Western Blotting
Enzyme-Linked Immunosorbent Assay
Lymphocytes
Transplants
Kidney

Keywords

  • AIDS
  • ELISA
  • False positivity
  • Haemodialysis
  • HIV infection

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Different types of false positive anti-HIV reactions in patients on haemodialysis. / Újhelyi, E.; Füst, G.; Illei, Gábor; Gyodi, Éva; Nagy, Károly; Tóth, F.; Büki, Béla; Dierich, Manfred P.; Mayer, Vlastimil; Gal, Gyorgy; Mako, János; Hollán, S.

In: Immunology Letters, Vol. 22, No. 1, 1989, p. 35-40.

Research output: Contribution to journalArticle

Újhelyi, E. ; Füst, G. ; Illei, Gábor ; Gyodi, Éva ; Nagy, Károly ; Tóth, F. ; Büki, Béla ; Dierich, Manfred P. ; Mayer, Vlastimil ; Gal, Gyorgy ; Mako, János ; Hollán, S. / Different types of false positive anti-HIV reactions in patients on haemodialysis. In: Immunology Letters. 1989 ; Vol. 22, No. 1. pp. 35-40.
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