Different sets of genes are activated by P53 upon UV or ionizing radiation in Drosophila melanogaster

Zsuzsanna Ujfaludi, I. Boros, E. Bálint

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The p53 tumour suppressor plays central role in the maintenance of genome integrity. P53 deficient fruit flies are highly sensitive to ionizing radiation (IR) and show genome instability suggesting that the Drosophila melanogaster p53 (Dmp53) is necessary for the proper damage response upon IR. We found that Dmp53 null fruit flies are highly sensitive to ultraviolet radiation (UV) as well. We analyzed the expression levels of apoptotic genes in wild type and Dmp53 null mutant animals after UV or IR using quantitative real-time RT-PCR. Ark (Apaf-1related killer) was induced in a Dmp53-dependent way upon UV treatment but not by IR, hid (head involution defective/ wrinkled) was induced upon both types of DNA damage, while reaper was induced only upon IR but not UV treatment. Using microarray analysis we identified several further genes that are activated upon UV irradiation in the presence of wild type Dmp53 only. Some but not all of these genes show Dmp53-dependent activation upon IR treatment as well. These results suggest that Dmp53 activates distinct cellular pathways through regulation of different target genes after different types of DNA damage.

Original languageEnglish
Pages (from-to)65-79
Number of pages15
JournalActa Biologica Hungarica
Volume58
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Ionizing radiation
p53 Genes
ultraviolet radiation
Ionizing Radiation
ionizing radiation
Drosophila melanogaster
Ultraviolet radiation
Genes
Radiation
gene
genes
Fruits
damage
fruit flies
Diptera
DNA damage
DNA Damage
genome
fruit
Fruit

Keywords

  • Ark
  • DNA damage
  • Drosophila melanogaster
  • p53
  • UV-C

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Different sets of genes are activated by P53 upon UV or ionizing radiation in Drosophila melanogaster. / Ujfaludi, Zsuzsanna; Boros, I.; Bálint, E.

In: Acta Biologica Hungarica, Vol. 58, No. SUPPL. 1, 11.2007, p. 65-79.

Research output: Contribution to journalArticle

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