1. 1. Carp and rabbit sarcoplasmatic reticulum Ca2+-ATPase enzymes were compared with respect to their sensitivity to FITC labelling. 2. 2. The carp enzyme showed much lower sensitivity to FITC in the Ca2+-Mg2+ activated ATPase activity. Fifty percent inhibition was observed at 20 μM labelling FITC concentration; in rabbit enzyme this inhibition was already achieved at 2 μM FITC. 3. 3. The tryptic cleavage products of the carp enzyme identified with immunoblot analysis as well as with FITC fluorescence, suggest multiple cleavage, yielding different fragments from the ones well known in rabbit and in rat enzyme. 4. 4. The present results indicates major structural differences with respect to the FITC binding, and tryptic cleavage between the SR Ca2+-ATPase enzymes from carp and rabbit, despite the cross-reactivity with polyclonal antibodies.
|Number of pages||5|
|Journal||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|Publication status||Published - May 1992|
ASJC Scopus subject areas
- Molecular Biology