Different pathways of human T-cell activation revealed by PHA-P and PHA-M

K. O'Flynn, M. Russul-Saib, I. Andó, D. L. Wallace, P. C. Beverley, A. W. Boylston, D. C. Linch

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Antigen-specific T-cell activation is mediated via the CD3-T(i) (antigen receptor) complex, and monoclonal antibodies to both CD3 and T(i) cause a rapid rise in intracellular Ca2+. This calcium mobilization is not inhibited by monoclonal antibodies to CD2. The rise in calcium mobilization induced by purified PHA (PHA-P) does not occur in a cell line which lacks CD2 expression, and can be blocked in other T cells by anti-CD2 antibodies. A combination of monoclonal antibodies to different epitopes of CD2 causes calcium mobilization and mitogenesis. Reagent grade PHA (PHA-M) induces calcium mobilization in cells that lack CD2, and its effects in other T cells cannot be blocked by anti-CD2 antibodies. The effects of PHA-P and PHA-M are thus mediated predominantly through different activation pathways.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
JournalImmunology
Volume57
Issue number1
Publication statusPublished - 1986

Fingerprint

Calcium
T-Lymphocytes
Monoclonal Antibodies
Anti-Idiotypic Antibodies
CD27 Antigens
Antigen Receptors
Viral Tumor Antigens
Epitopes
Cell Line
phytohemagglutinin-P
phytohemagglutinin-M

ASJC Scopus subject areas

  • Immunology

Cite this

O'Flynn, K., Russul-Saib, M., Andó, I., Wallace, D. L., Beverley, P. C., Boylston, A. W., & Linch, D. C. (1986). Different pathways of human T-cell activation revealed by PHA-P and PHA-M. Immunology, 57(1), 55-60.

Different pathways of human T-cell activation revealed by PHA-P and PHA-M. / O'Flynn, K.; Russul-Saib, M.; Andó, I.; Wallace, D. L.; Beverley, P. C.; Boylston, A. W.; Linch, D. C.

In: Immunology, Vol. 57, No. 1, 1986, p. 55-60.

Research output: Contribution to journalArticle

O'Flynn, K, Russul-Saib, M, Andó, I, Wallace, DL, Beverley, PC, Boylston, AW & Linch, DC 1986, 'Different pathways of human T-cell activation revealed by PHA-P and PHA-M', Immunology, vol. 57, no. 1, pp. 55-60.
O'Flynn K, Russul-Saib M, Andó I, Wallace DL, Beverley PC, Boylston AW et al. Different pathways of human T-cell activation revealed by PHA-P and PHA-M. Immunology. 1986;57(1):55-60.
O'Flynn, K. ; Russul-Saib, M. ; Andó, I. ; Wallace, D. L. ; Beverley, P. C. ; Boylston, A. W. ; Linch, D. C. / Different pathways of human T-cell activation revealed by PHA-P and PHA-M. In: Immunology. 1986 ; Vol. 57, No. 1. pp. 55-60.
@article{c7785a1b0b254c62a745b403c55d0eeb,
title = "Different pathways of human T-cell activation revealed by PHA-P and PHA-M",
abstract = "Antigen-specific T-cell activation is mediated via the CD3-T(i) (antigen receptor) complex, and monoclonal antibodies to both CD3 and T(i) cause a rapid rise in intracellular Ca2+. This calcium mobilization is not inhibited by monoclonal antibodies to CD2. The rise in calcium mobilization induced by purified PHA (PHA-P) does not occur in a cell line which lacks CD2 expression, and can be blocked in other T cells by anti-CD2 antibodies. A combination of monoclonal antibodies to different epitopes of CD2 causes calcium mobilization and mitogenesis. Reagent grade PHA (PHA-M) induces calcium mobilization in cells that lack CD2, and its effects in other T cells cannot be blocked by anti-CD2 antibodies. The effects of PHA-P and PHA-M are thus mediated predominantly through different activation pathways.",
author = "K. O'Flynn and M. Russul-Saib and I. And{\'o} and Wallace, {D. L.} and Beverley, {P. C.} and Boylston, {A. W.} and Linch, {D. C.}",
year = "1986",
language = "English",
volume = "57",
pages = "55--60",
journal = "Immunology",
issn = "0019-2805",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Different pathways of human T-cell activation revealed by PHA-P and PHA-M

AU - O'Flynn, K.

AU - Russul-Saib, M.

AU - Andó, I.

AU - Wallace, D. L.

AU - Beverley, P. C.

AU - Boylston, A. W.

AU - Linch, D. C.

PY - 1986

Y1 - 1986

N2 - Antigen-specific T-cell activation is mediated via the CD3-T(i) (antigen receptor) complex, and monoclonal antibodies to both CD3 and T(i) cause a rapid rise in intracellular Ca2+. This calcium mobilization is not inhibited by monoclonal antibodies to CD2. The rise in calcium mobilization induced by purified PHA (PHA-P) does not occur in a cell line which lacks CD2 expression, and can be blocked in other T cells by anti-CD2 antibodies. A combination of monoclonal antibodies to different epitopes of CD2 causes calcium mobilization and mitogenesis. Reagent grade PHA (PHA-M) induces calcium mobilization in cells that lack CD2, and its effects in other T cells cannot be blocked by anti-CD2 antibodies. The effects of PHA-P and PHA-M are thus mediated predominantly through different activation pathways.

AB - Antigen-specific T-cell activation is mediated via the CD3-T(i) (antigen receptor) complex, and monoclonal antibodies to both CD3 and T(i) cause a rapid rise in intracellular Ca2+. This calcium mobilization is not inhibited by monoclonal antibodies to CD2. The rise in calcium mobilization induced by purified PHA (PHA-P) does not occur in a cell line which lacks CD2 expression, and can be blocked in other T cells by anti-CD2 antibodies. A combination of monoclonal antibodies to different epitopes of CD2 causes calcium mobilization and mitogenesis. Reagent grade PHA (PHA-M) induces calcium mobilization in cells that lack CD2, and its effects in other T cells cannot be blocked by anti-CD2 antibodies. The effects of PHA-P and PHA-M are thus mediated predominantly through different activation pathways.

UR - http://www.scopus.com/inward/record.url?scp=0022643125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022643125&partnerID=8YFLogxK

M3 - Article

VL - 57

SP - 55

EP - 60

JO - Immunology

JF - Immunology

SN - 0019-2805

IS - 1

ER -