Different pathways of human T-cell activation revealed by PHA-P and PHA-M

K. O'Flynn, M. Russul-Saib, I. Ando, D. L. Wallace, P. C. Beverley, A. W. Boylston, D. C. Linch

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Abstract

Antigen-specific T-cell activation is mediated via the CD3-T(i) (antigen receptor) complex, and monoclonal antibodies to both CD3 and T(i) cause a rapid rise in intracellular Ca2+. This calcium mobilization is not inhibited by monoclonal antibodies to CD2. The rise in calcium mobilization induced by purified PHA (PHA-P) does not occur in a cell line which lacks CD2 expression, and can be blocked in other T cells by anti-CD2 antibodies. A combination of monoclonal antibodies to different epitopes of CD2 causes calcium mobilization and mitogenesis. Reagent grade PHA (PHA-M) induces calcium mobilization in cells that lack CD2, and its effects in other T cells cannot be blocked by anti-CD2 antibodies. The effects of PHA-P and PHA-M are thus mediated predominantly through different activation pathways.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
JournalImmunology
Volume57
Issue number1
Publication statusPublished - Mar 7 1986

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

O'Flynn, K., Russul-Saib, M., Ando, I., Wallace, D. L., Beverley, P. C., Boylston, A. W., & Linch, D. C. (1986). Different pathways of human T-cell activation revealed by PHA-P and PHA-M. Immunology, 57(1), 55-60.