Different isoforms of PRIP-interacting protein with methyltransferase domain/trimethylguanosine synthase localizes to the cytoplasm and nucleus

Izzet Enünlü, Gábor Pápai, Imre Cserpán, A. Udvardy, Kuan Teh Jeang, I. Boros

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A protein family including the recently identified PIMT/Tgs1 (PRIP-interacting protein with methyltransferase domain/trimethylguanosine synthase) was identified by searching databases for homologues of a newly identified Drosophila protein with RNA-binding activity and methyltransferase domain. Antibodies raised against a short peptide of the mammalian homologue show a 90-kDa isoform expressed specifically in rat brain and testis and a 55-kDa form expressed ubiquitously. In HeLa cells, the larger isoform of the protein is nuclear and associated with a 600-kDa complex, while the smaller isoform is mainly cytoplasmic and co-localizes to the tubulin network. Inhibition of PIMT/Tgs1 expression by siRNA in HeLa cells resulted in an increase in the percentage of cells in G2/M phases. In yeast two-hybrid and in vitro GST pull down experiments, the conserved C-terminal region of PIMT/Tgs1 interacted with the WD domain containing EED/WAIT-1 that acts as a polycomb-type repressor in the nucleus and also binds to integrins in the cytoplasm. Our experiments, together with earlier data, indicate that isoforms of the PIMT/Tgs1 protein with an RNA methyltransferase domain function both in the nucleus and in the cytoplasm and associate with both elements of the cytoskeletal network and nuclear factors known to be involved in gene regulation.

Original languageEnglish
Pages (from-to)44-51
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume309
Issue number1
DOIs
Publication statusPublished - Sep 12 2003

Fingerprint

Protein Methyltransferases
Methyltransferases
Protein Isoforms
Cytoplasm
HeLa Cells
Proteins
RNA
Drosophila Proteins
G2 Phase
Tubulin
Nuclear Proteins
Gene expression
Integrins
Cell Division
Yeast
Small Interfering RNA
Testis
Rats
Brain
Yeasts

Keywords

  • Cytoskeleton
  • DTL
  • Methyltransferase
  • PIMT
  • Tgs1
  • WAIT-1
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Different isoforms of PRIP-interacting protein with methyltransferase domain/trimethylguanosine synthase localizes to the cytoplasm and nucleus. / Enünlü, Izzet; Pápai, Gábor; Cserpán, Imre; Udvardy, A.; Jeang, Kuan Teh; Boros, I.

In: Biochemical and Biophysical Research Communications, Vol. 309, No. 1, 12.09.2003, p. 44-51.

Research output: Contribution to journalArticle

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