Different electrophysiological actions of 24- and 72-hour aggregated amyloid-beta oligomers on hippocampal field population spike in both anesthetized and awake rats

Gergely Orbán, Katalin Völgyi, G. Juhász, B. Penke, K. Kékesi, J. Kardos, A. Czurkó

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13 Citations (Scopus)

Abstract

Diffusible oligomeric assemblies of the amyloid β-protein (Aβ) could be the primary factor in the pathogenic pathway leading to Alzheimer's disease (AD). Converging lines of evidence support the notion that AD begins with subtle alterations in synaptic efficacy, prior to the occurrence of extensive neuronal degeneration. Recently, however, a shared or overlapping pathogenesis for AD and epileptic seizures occurred as aberrant neuronal hyperexcitability, as well as nonconvulsive seizure activity were found in several different APP transgenic mouse lines. This generated a renewed attention to the well-known comorbidity of AD and epilepsy and interest in how Aβ oligomers influence neuronal excitability. In this study therefore, we investigated the effect of various in vitro-aged Aβ(1-42) oligomer solutions on the perforant pathway-evoked field potentials in the ventral hippocampal dentate gyrus in vivo. Firstly, Aβ oligomer solutions (1 μl, 200 μM) which had been aggregated in vitro for 0, 24 or 72 h were injected into the hippocampus of urethane-anesthetized rats, in parallel with in vitro physico-chemical characterization of Aβ oligomerization (atomic force microscopy, thioflavin-T fluorescence). We found a marked increase of hippocampal population spike (pSpike) after injection of the 24-h Aβ oligomer solution and a decrease of the pSpike amplitude after injection of the 72-h Aβ oligomer. Since urethane anesthesia affects the properties of hippocampal evoked potentials, we repeated the injection of these two Aβ oligomer solutions in awake, freely moving animals. Evoked responses to perforant pathway stimulation revealed a 70% increase of pSpike amplitude 50 min after the 24-h Aβ oligomer injection and a 55% decrease after the 72-h Aβ oligomer injection. Field potentials, that reflect synaptic potentials, were not affected by the Aβ injection. These results demonstrate that oligomeric Aβ aggregates elicit opposite electrophysiological effects on neuronal excitability which depend on their degree of oligomerization.

Original languageEnglish
Pages (from-to)227-235
Number of pages9
JournalBrain Research
Volume1354
DOIs
Publication statusPublished - Oct 1 2010

Fingerprint

Amyloid
Injections
Alzheimer Disease
Population
Perforant Pathway
Urethane
Evoked Potentials
Epilepsy
Serum Amyloid A Protein
Parahippocampal Gyrus
Synaptic Potentials
Atomic Force Microscopy
Dentate Gyrus
Population Dynamics
Transgenic Mice
Comorbidity
Hippocampus
Seizures
Anesthesia
Fluorescence

Keywords

  • Alzheimer's disease
  • Amyloid
  • Atomic force microscopy
  • Excitability
  • Oligomer

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

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title = "Different electrophysiological actions of 24- and 72-hour aggregated amyloid-beta oligomers on hippocampal field population spike in both anesthetized and awake rats",
abstract = "Diffusible oligomeric assemblies of the amyloid β-protein (Aβ) could be the primary factor in the pathogenic pathway leading to Alzheimer's disease (AD). Converging lines of evidence support the notion that AD begins with subtle alterations in synaptic efficacy, prior to the occurrence of extensive neuronal degeneration. Recently, however, a shared or overlapping pathogenesis for AD and epileptic seizures occurred as aberrant neuronal hyperexcitability, as well as nonconvulsive seizure activity were found in several different APP transgenic mouse lines. This generated a renewed attention to the well-known comorbidity of AD and epilepsy and interest in how Aβ oligomers influence neuronal excitability. In this study therefore, we investigated the effect of various in vitro-aged Aβ(1-42) oligomer solutions on the perforant pathway-evoked field potentials in the ventral hippocampal dentate gyrus in vivo. Firstly, Aβ oligomer solutions (1 μl, 200 μM) which had been aggregated in vitro for 0, 24 or 72 h were injected into the hippocampus of urethane-anesthetized rats, in parallel with in vitro physico-chemical characterization of Aβ oligomerization (atomic force microscopy, thioflavin-T fluorescence). We found a marked increase of hippocampal population spike (pSpike) after injection of the 24-h Aβ oligomer solution and a decrease of the pSpike amplitude after injection of the 72-h Aβ oligomer. Since urethane anesthesia affects the properties of hippocampal evoked potentials, we repeated the injection of these two Aβ oligomer solutions in awake, freely moving animals. Evoked responses to perforant pathway stimulation revealed a 70{\%} increase of pSpike amplitude 50 min after the 24-h Aβ oligomer injection and a 55{\%} decrease after the 72-h Aβ oligomer injection. Field potentials, that reflect synaptic potentials, were not affected by the Aβ injection. These results demonstrate that oligomeric Aβ aggregates elicit opposite electrophysiological effects on neuronal excitability which depend on their degree of oligomerization.",
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T1 - Different electrophysiological actions of 24- and 72-hour aggregated amyloid-beta oligomers on hippocampal field population spike in both anesthetized and awake rats

AU - Orbán, Gergely

AU - Völgyi, Katalin

AU - Juhász, G.

AU - Penke, B.

AU - Kékesi, K.

AU - Kardos, J.

AU - Czurkó, A.

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