Different effects of endothelin-1 on calcium and potassium currents in canine ventricular cells

T. Bányász, J. Magyar, Á Körtvély, G. Szigeti, P. Szigligeti, Z. Papp, A. Mohácsi, L. Kovács, P. Nánási

Research output: Contribution to journalArticle

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Abstract

Effects of endothelin-1 (ET-1) on the L-type calcium current (ICa) and delayed rectifier potassium current (IK) were studied in isolated canine ventricular cardiomyocytes using the whole-cell configuration of the patch-clamp technique. ET-1 (8 nM) was applied in three experimental arrangements: untreated cells, in the presence of 50 nM isoproterenol, and in the presence of 250 μM 8-bromo-cAMP. In untreated cells, ET-1 significantly decreased the peak amplitude of ICa by 32.3±4.8% at +5 mV PCa. ET-1 had no effect on the amplitude of IK, Ito (transient outward current) or IK1 (inward rectifier K current) in untreated cells; however, the time course of recovery from inactivation of Ito was significantly increased by ET-1 (from 26.5±4.6 ms to 59.5±1.8 ms, PCa to 263±29% of control. ET-1 reduced ICa also in isoproterenol-treated cells by 17.8±2% (PK was increased by isoproterenol to 213±18% of control. This effect of isoproterenol on IK was reduced by 31.8±4.8% if the cells were pretreated with ET-1. Similarly, in isoproterenol-treated cells ET-1 decreased IK by 16.2±1.5% (PCa or IK. It was concluded that differences)in effects of ET-1 on ICa and IK may be related to differences in cAMP sensitivity of the currents.

Original languageEnglish
Pages (from-to)383-390
Number of pages8
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume363
Issue number4
DOIs
Publication statusPublished - 2001

Fingerprint

Endothelin-1
Canidae
Potassium
Calcium
Isoproterenol
8-Bromo Cyclic Adenosine Monophosphate
Patch-Clamp Techniques
Cardiac Myocytes

Keywords

  • Action potential
  • Calcium current
  • cAMP
  • Cardiac cells
  • Endothelin
  • Potassium currents
  • Signal transduction

ASJC Scopus subject areas

  • Pharmacology

Cite this

Different effects of endothelin-1 on calcium and potassium currents in canine ventricular cells. / Bányász, T.; Magyar, J.; Körtvély, Á; Szigeti, G.; Szigligeti, P.; Papp, Z.; Mohácsi, A.; Kovács, L.; Nánási, P.

In: Naunyn-Schmiedeberg's Archives of Pharmacology, Vol. 363, No. 4, 2001, p. 383-390.

Research output: Contribution to journalArticle

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abstract = "Effects of endothelin-1 (ET-1) on the L-type calcium current (ICa) and delayed rectifier potassium current (IK) were studied in isolated canine ventricular cardiomyocytes using the whole-cell configuration of the patch-clamp technique. ET-1 (8 nM) was applied in three experimental arrangements: untreated cells, in the presence of 50 nM isoproterenol, and in the presence of 250 μM 8-bromo-cAMP. In untreated cells, ET-1 significantly decreased the peak amplitude of ICa by 32.3±4.8{\%} at +5 mV PCa. ET-1 had no effect on the amplitude of IK, Ito (transient outward current) or IK1 (inward rectifier K current) in untreated cells; however, the time course of recovery from inactivation of Ito was significantly increased by ET-1 (from 26.5±4.6 ms to 59.5±1.8 ms, PCa to 263±29{\%} of control. ET-1 reduced ICa also in isoproterenol-treated cells by 17.8±2{\%} (PK was increased by isoproterenol to 213±18{\%} of control. This effect of isoproterenol on IK was reduced by 31.8±4.8{\%} if the cells were pretreated with ET-1. Similarly, in isoproterenol-treated cells ET-1 decreased IK by 16.2±1.5{\%} (PCa or IK. It was concluded that differences)in effects of ET-1 on ICa and IK may be related to differences in cAMP sensitivity of the currents.",
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AU - Bányász, T.

AU - Magyar, J.

AU - Körtvély, Á

AU - Szigeti, G.

AU - Szigligeti, P.

AU - Papp, Z.

AU - Mohácsi, A.

AU - Kovács, L.

AU - Nánási, P.

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N2 - Effects of endothelin-1 (ET-1) on the L-type calcium current (ICa) and delayed rectifier potassium current (IK) were studied in isolated canine ventricular cardiomyocytes using the whole-cell configuration of the patch-clamp technique. ET-1 (8 nM) was applied in three experimental arrangements: untreated cells, in the presence of 50 nM isoproterenol, and in the presence of 250 μM 8-bromo-cAMP. In untreated cells, ET-1 significantly decreased the peak amplitude of ICa by 32.3±4.8% at +5 mV PCa. ET-1 had no effect on the amplitude of IK, Ito (transient outward current) or IK1 (inward rectifier K current) in untreated cells; however, the time course of recovery from inactivation of Ito was significantly increased by ET-1 (from 26.5±4.6 ms to 59.5±1.8 ms, PCa to 263±29% of control. ET-1 reduced ICa also in isoproterenol-treated cells by 17.8±2% (PK was increased by isoproterenol to 213±18% of control. This effect of isoproterenol on IK was reduced by 31.8±4.8% if the cells were pretreated with ET-1. Similarly, in isoproterenol-treated cells ET-1 decreased IK by 16.2±1.5% (PCa or IK. It was concluded that differences)in effects of ET-1 on ICa and IK may be related to differences in cAMP sensitivity of the currents.

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