Different effects of an oligonucleotide uptake stimulating protein on leukemic cells in their primitive and differentiated state

György Tóth, József Schlammadinger, S. Sipka, Attila Kiss, G. Szegedi, Kálmán Rák, Gábor Szabó

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The single stranded [3H]oligonucleotide uptake by H L-60 human promyelocyte and K562 human erythroleukemia cells was stimulated 20-45-fold by DUSF (DNA uptake stimulating protein), and this effect was drastically reduced (to 1.6-13X) if the cells were induced to differentiate. The oligonucleotide uptake stimulating effect of DUSF was not altered in HL-60 and K562 cells, if the proliferation of the cells was inhibited by hydroxyurea (HU) treatment. The oligonucleotide uptake by separated granulocytes and mononuclear cells from healthy donors was not stimulated by DUSF, while the uptake of oligonucleotides by myeloid and lymphoid leukemic cells was greatly stimulated (10-15X). The uptake of oligonucleotides by differentiated mononuclear cells of healthy donors could not be stimulated by DUSF, but the oligonucleotide uptake was greatly increased (11X) by DUSF if the cells were subjected to blast transformation.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalFEBS Letters
Volume430
Issue number3
DOIs
Publication statusPublished - Jul 3 1998

Fingerprint

Oligonucleotides
Proteins
Granulocyte Precursor Cells
Leukemia, Erythroblastic, Acute
Hydroxyurea
K562 Cells
HL-60 Cells
Lymphocyte Activation
Granulocytes
Cell Proliferation
Lymphocytes
DNA

Keywords

  • Antisense oligonucleotide
  • Cell proliferation
  • DNA uptake stimulating protein
  • Leukemia
  • Oligonucleotide uptake

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Different effects of an oligonucleotide uptake stimulating protein on leukemic cells in their primitive and differentiated state. / Tóth, György; Schlammadinger, József; Sipka, S.; Kiss, Attila; Szegedi, G.; Rák, Kálmán; Szabó, Gábor.

In: FEBS Letters, Vol. 430, No. 3, 03.07.1998, p. 241-245.

Research output: Contribution to journalArticle

Tóth, György ; Schlammadinger, József ; Sipka, S. ; Kiss, Attila ; Szegedi, G. ; Rák, Kálmán ; Szabó, Gábor. / Different effects of an oligonucleotide uptake stimulating protein on leukemic cells in their primitive and differentiated state. In: FEBS Letters. 1998 ; Vol. 430, No. 3. pp. 241-245.
@article{fce9ec00272c4cadbc7ab62e9ee83567,
title = "Different effects of an oligonucleotide uptake stimulating protein on leukemic cells in their primitive and differentiated state",
abstract = "The single stranded [3H]oligonucleotide uptake by H L-60 human promyelocyte and K562 human erythroleukemia cells was stimulated 20-45-fold by DUSF (DNA uptake stimulating protein), and this effect was drastically reduced (to 1.6-13X) if the cells were induced to differentiate. The oligonucleotide uptake stimulating effect of DUSF was not altered in HL-60 and K562 cells, if the proliferation of the cells was inhibited by hydroxyurea (HU) treatment. The oligonucleotide uptake by separated granulocytes and mononuclear cells from healthy donors was not stimulated by DUSF, while the uptake of oligonucleotides by myeloid and lymphoid leukemic cells was greatly stimulated (10-15X). The uptake of oligonucleotides by differentiated mononuclear cells of healthy donors could not be stimulated by DUSF, but the oligonucleotide uptake was greatly increased (11X) by DUSF if the cells were subjected to blast transformation.",
keywords = "Antisense oligonucleotide, Cell proliferation, DNA uptake stimulating protein, Leukemia, Oligonucleotide uptake",
author = "Gy{\"o}rgy T{\'o}th and J{\'o}zsef Schlammadinger and S. Sipka and Attila Kiss and G. Szegedi and K{\'a}lm{\'a}n R{\'a}k and G{\'a}bor Szab{\'o}",
year = "1998",
month = "7",
day = "3",
doi = "10.1016/S0014-5793(98)00663-2",
language = "English",
volume = "430",
pages = "241--245",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Different effects of an oligonucleotide uptake stimulating protein on leukemic cells in their primitive and differentiated state

AU - Tóth, György

AU - Schlammadinger, József

AU - Sipka, S.

AU - Kiss, Attila

AU - Szegedi, G.

AU - Rák, Kálmán

AU - Szabó, Gábor

PY - 1998/7/3

Y1 - 1998/7/3

N2 - The single stranded [3H]oligonucleotide uptake by H L-60 human promyelocyte and K562 human erythroleukemia cells was stimulated 20-45-fold by DUSF (DNA uptake stimulating protein), and this effect was drastically reduced (to 1.6-13X) if the cells were induced to differentiate. The oligonucleotide uptake stimulating effect of DUSF was not altered in HL-60 and K562 cells, if the proliferation of the cells was inhibited by hydroxyurea (HU) treatment. The oligonucleotide uptake by separated granulocytes and mononuclear cells from healthy donors was not stimulated by DUSF, while the uptake of oligonucleotides by myeloid and lymphoid leukemic cells was greatly stimulated (10-15X). The uptake of oligonucleotides by differentiated mononuclear cells of healthy donors could not be stimulated by DUSF, but the oligonucleotide uptake was greatly increased (11X) by DUSF if the cells were subjected to blast transformation.

AB - The single stranded [3H]oligonucleotide uptake by H L-60 human promyelocyte and K562 human erythroleukemia cells was stimulated 20-45-fold by DUSF (DNA uptake stimulating protein), and this effect was drastically reduced (to 1.6-13X) if the cells were induced to differentiate. The oligonucleotide uptake stimulating effect of DUSF was not altered in HL-60 and K562 cells, if the proliferation of the cells was inhibited by hydroxyurea (HU) treatment. The oligonucleotide uptake by separated granulocytes and mononuclear cells from healthy donors was not stimulated by DUSF, while the uptake of oligonucleotides by myeloid and lymphoid leukemic cells was greatly stimulated (10-15X). The uptake of oligonucleotides by differentiated mononuclear cells of healthy donors could not be stimulated by DUSF, but the oligonucleotide uptake was greatly increased (11X) by DUSF if the cells were subjected to blast transformation.

KW - Antisense oligonucleotide

KW - Cell proliferation

KW - DNA uptake stimulating protein

KW - Leukemia

KW - Oligonucleotide uptake

UR - http://www.scopus.com/inward/record.url?scp=0032479209&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032479209&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(98)00663-2

DO - 10.1016/S0014-5793(98)00663-2

M3 - Article

VL - 430

SP - 241

EP - 245

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -