Different actions of secretin and Gly-extended secretin predict secretin receptor subtypes

Travis E. Solomon, G. Varga, Ning Zeng, S. Vincent Wu, John H. Walsht, Joseph R. Reeve

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Only one secretin receptor has been cloned and its properties characterized in native and transfected cells. To test the hypothesis that stimulatory and inhibitory effects of secretin are mediated by different secretin receptor subtypes, pancreatic and gastric secretory responses to secretin and secretin-Gly were determined in rats. Pancreatic fluid secretion was increased equipotently by secretin and secretin-Gly, but secretin was markedly more potent for inhibition of basal and gastrin-induced acid secretion. In Chinese hamster ovary cells stably transfected with the rat secretin receptor, secretin and secretin-Gly equipotently displaced 125I-labeled secretin (IC50 values 5.3 ± 0.5 and 6.4 ± 0.6 nM, respectively). Secretin, but not secretin-Gly, caused release of somatostatin from rat gastric mucosal D cells. Thus the equipotent actions of secretin and secretin-Gly on pancreatic secretion appear to result from equal binding and activation of the pancreatic secretin receptor. Conversely, secretin more potently inhibited gastric acid secretion in vivo, and only secretin released somatostatin from D cells in vitro. These results support the existence of a secretin receptor subtype mediating inhibition of gastric acid secretion that is distinct from the previously characterized pancreatic secretin receptor.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume280
Issue number1 43-1
Publication statusPublished - Jan 2001

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Secretin
Somatostatin-Secreting Cells
Gastric Acid
secretin receptor
Fluids and Secretions
Gastrins
Cricetulus
Somatostatin
Inhibitory Concentration 50

Keywords

  • D cells
  • Posttranslational processing
  • Receptor specificity
  • Somatostatin

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

Cite this

Different actions of secretin and Gly-extended secretin predict secretin receptor subtypes. / Solomon, Travis E.; Varga, G.; Zeng, Ning; Wu, S. Vincent; Walsht, John H.; Reeve, Joseph R.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 280, No. 1 43-1, 01.2001.

Research output: Contribution to journalArticle

Solomon, Travis E. ; Varga, G. ; Zeng, Ning ; Wu, S. Vincent ; Walsht, John H. ; Reeve, Joseph R. / Different actions of secretin and Gly-extended secretin predict secretin receptor subtypes. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2001 ; Vol. 280, No. 1 43-1.
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