Differences in the Lipid Composition of Plasma Membrane in Two Near-isogenic Lines of Wheat (SrHopeRa and SrHopeSa) Differing in their Resistance to Stem Rust

F. Dubert, A. Skoczowski, B. Barna, E. Niemczyk

Research output: Contribution to journalArticle

Abstract

The composition of fatty acids of phospho- (PL) and glycolipids (GL) and sterols (ST) of plasma membranes prepared by phase separation from microsomal fractions from leaves of two near-isogenic wheat lines, differing in their resistance to stem rust (the resistant line-SrHopeRa, and the sensitive line SrHopeSa) was investigated. In the PL fraction palmitic, linoleic and linolenic acids, constituting about 90% of all fatty acids. Smaller amounts of stearic and linoleic acids and traces of palmitooleic acids were also found. The pathogen resistant genotype (Ra) was characterised by a greater amount of poly-unsaturated (linoleic and linolenic) fatty acids. In the GL fraction the main fatty acids were palmitic acid (over 40%), oleic, linoleic and linolenic acids (over 50% of all the fatty acids). The stearic acid occurred in small, and the palmitooleic acid - in trace amounts. The Ra genotype showed a smaller amount of the oleic acid and a higher content of the linolenic acid. The comparison of indicators describing unsaturation level of plasma membrane fatty acids (U/S, DBI) exhibited higher values for Ra genotype as compared to SA one, particularly in PL and DG fractions. Both genotypes showed also some differences hi plasma membrane sterol composition. These differences resulted in smaller value of MPS/LPS ratio in Ra genotype indicating higher fluidity of the plasma membrane in this genotype. It is assumed that the higher pathogen resistance of the Ra genotype may be related to the higher fluidity level of its plasma membrane. List of abbreviations: 16:0 - palmitic acid; 16:1 - palmitooleic acid; 18:0 - stearic acid; 18:1 - oleic acid; 18:2 - linoleic acid; 18:3 - linolenic acid; 18:1/18:0 and 18:3/18:2 - ratio of the appropriate fatty acids content; CHOLE - cholesterol; CAMPE - campesterol; SITO - ß-sitosterol; ST - sterols; STIGMA - stigmasterol; DG - digalactosyl diacylgilcerol; MG - monogalactosyl diacylglycerol; LPS - less planar sterols (stigmasterol and sitosterol); MPS - more planar sterols (cholesterol and campesterol); LPS/MPS - ratio of the appropriate sterols content; PL - phospholipids; U/S - ratio of unsaturated to saturated fatty acids; DBI - double bond index.

Original languageEnglish
Pages (from-to)243-250
Number of pages8
JournalActa Phytopathologica et Entomologica Hungarica
Volume35
Issue number1
Publication statusPublished - 2000

Fingerprint

stem rust
sitosterols
stigmasterol
isogenic lines
lipid composition
plasma membrane
linolenic acid
wheat
genotype
linoleic acid
stearic acid
palmitic acid
fatty acids
oleic acid
campesterol
glycolipids
acids
fatty acid composition
cholesterol
pathogens

Keywords

  • Fatty acids
  • Galactolipids
  • Near-isogenic lines
  • Phospholipids
  • Plasmalemma
  • Rust
  • Sterols
  • Wheat

ASJC Scopus subject areas

  • Insect Science
  • Plant Science

Cite this

@article{c128e83f0cb744f3926a187af503c83f,
title = "Differences in the Lipid Composition of Plasma Membrane in Two Near-isogenic Lines of Wheat (SrHopeRa and SrHopeSa) Differing in their Resistance to Stem Rust",
abstract = "The composition of fatty acids of phospho- (PL) and glycolipids (GL) and sterols (ST) of plasma membranes prepared by phase separation from microsomal fractions from leaves of two near-isogenic wheat lines, differing in their resistance to stem rust (the resistant line-SrHopeRa, and the sensitive line SrHopeSa) was investigated. In the PL fraction palmitic, linoleic and linolenic acids, constituting about 90{\%} of all fatty acids. Smaller amounts of stearic and linoleic acids and traces of palmitooleic acids were also found. The pathogen resistant genotype (Ra) was characterised by a greater amount of poly-unsaturated (linoleic and linolenic) fatty acids. In the GL fraction the main fatty acids were palmitic acid (over 40{\%}), oleic, linoleic and linolenic acids (over 50{\%} of all the fatty acids). The stearic acid occurred in small, and the palmitooleic acid - in trace amounts. The Ra genotype showed a smaller amount of the oleic acid and a higher content of the linolenic acid. The comparison of indicators describing unsaturation level of plasma membrane fatty acids (U/S, DBI) exhibited higher values for Ra genotype as compared to SA one, particularly in PL and DG fractions. Both genotypes showed also some differences hi plasma membrane sterol composition. These differences resulted in smaller value of MPS/LPS ratio in Ra genotype indicating higher fluidity of the plasma membrane in this genotype. It is assumed that the higher pathogen resistance of the Ra genotype may be related to the higher fluidity level of its plasma membrane. List of abbreviations: 16:0 - palmitic acid; 16:1 - palmitooleic acid; 18:0 - stearic acid; 18:1 - oleic acid; 18:2 - linoleic acid; 18:3 - linolenic acid; 18:1/18:0 and 18:3/18:2 - ratio of the appropriate fatty acids content; CHOLE - cholesterol; CAMPE - campesterol; SITO - {\ss}-sitosterol; ST - sterols; STIGMA - stigmasterol; DG - digalactosyl diacylgilcerol; MG - monogalactosyl diacylglycerol; LPS - less planar sterols (stigmasterol and sitosterol); MPS - more planar sterols (cholesterol and campesterol); LPS/MPS - ratio of the appropriate sterols content; PL - phospholipids; U/S - ratio of unsaturated to saturated fatty acids; DBI - double bond index.",
keywords = "Fatty acids, Galactolipids, Near-isogenic lines, Phospholipids, Plasmalemma, Rust, Sterols, Wheat",
author = "F. Dubert and A. Skoczowski and B. Barna and E. Niemczyk",
year = "2000",
language = "English",
volume = "35",
pages = "243--250",
journal = "Acta Phytopathologica et Entomologica Hungarica",
issn = "0238-1249",
publisher = "Akademiai Kiado",
number = "1",

}

TY - JOUR

T1 - Differences in the Lipid Composition of Plasma Membrane in Two Near-isogenic Lines of Wheat (SrHopeRa and SrHopeSa) Differing in their Resistance to Stem Rust

AU - Dubert, F.

AU - Skoczowski, A.

AU - Barna, B.

AU - Niemczyk, E.

PY - 2000

Y1 - 2000

N2 - The composition of fatty acids of phospho- (PL) and glycolipids (GL) and sterols (ST) of plasma membranes prepared by phase separation from microsomal fractions from leaves of two near-isogenic wheat lines, differing in their resistance to stem rust (the resistant line-SrHopeRa, and the sensitive line SrHopeSa) was investigated. In the PL fraction palmitic, linoleic and linolenic acids, constituting about 90% of all fatty acids. Smaller amounts of stearic and linoleic acids and traces of palmitooleic acids were also found. The pathogen resistant genotype (Ra) was characterised by a greater amount of poly-unsaturated (linoleic and linolenic) fatty acids. In the GL fraction the main fatty acids were palmitic acid (over 40%), oleic, linoleic and linolenic acids (over 50% of all the fatty acids). The stearic acid occurred in small, and the palmitooleic acid - in trace amounts. The Ra genotype showed a smaller amount of the oleic acid and a higher content of the linolenic acid. The comparison of indicators describing unsaturation level of plasma membrane fatty acids (U/S, DBI) exhibited higher values for Ra genotype as compared to SA one, particularly in PL and DG fractions. Both genotypes showed also some differences hi plasma membrane sterol composition. These differences resulted in smaller value of MPS/LPS ratio in Ra genotype indicating higher fluidity of the plasma membrane in this genotype. It is assumed that the higher pathogen resistance of the Ra genotype may be related to the higher fluidity level of its plasma membrane. List of abbreviations: 16:0 - palmitic acid; 16:1 - palmitooleic acid; 18:0 - stearic acid; 18:1 - oleic acid; 18:2 - linoleic acid; 18:3 - linolenic acid; 18:1/18:0 and 18:3/18:2 - ratio of the appropriate fatty acids content; CHOLE - cholesterol; CAMPE - campesterol; SITO - ß-sitosterol; ST - sterols; STIGMA - stigmasterol; DG - digalactosyl diacylgilcerol; MG - monogalactosyl diacylglycerol; LPS - less planar sterols (stigmasterol and sitosterol); MPS - more planar sterols (cholesterol and campesterol); LPS/MPS - ratio of the appropriate sterols content; PL - phospholipids; U/S - ratio of unsaturated to saturated fatty acids; DBI - double bond index.

AB - The composition of fatty acids of phospho- (PL) and glycolipids (GL) and sterols (ST) of plasma membranes prepared by phase separation from microsomal fractions from leaves of two near-isogenic wheat lines, differing in their resistance to stem rust (the resistant line-SrHopeRa, and the sensitive line SrHopeSa) was investigated. In the PL fraction palmitic, linoleic and linolenic acids, constituting about 90% of all fatty acids. Smaller amounts of stearic and linoleic acids and traces of palmitooleic acids were also found. The pathogen resistant genotype (Ra) was characterised by a greater amount of poly-unsaturated (linoleic and linolenic) fatty acids. In the GL fraction the main fatty acids were palmitic acid (over 40%), oleic, linoleic and linolenic acids (over 50% of all the fatty acids). The stearic acid occurred in small, and the palmitooleic acid - in trace amounts. The Ra genotype showed a smaller amount of the oleic acid and a higher content of the linolenic acid. The comparison of indicators describing unsaturation level of plasma membrane fatty acids (U/S, DBI) exhibited higher values for Ra genotype as compared to SA one, particularly in PL and DG fractions. Both genotypes showed also some differences hi plasma membrane sterol composition. These differences resulted in smaller value of MPS/LPS ratio in Ra genotype indicating higher fluidity of the plasma membrane in this genotype. It is assumed that the higher pathogen resistance of the Ra genotype may be related to the higher fluidity level of its plasma membrane. List of abbreviations: 16:0 - palmitic acid; 16:1 - palmitooleic acid; 18:0 - stearic acid; 18:1 - oleic acid; 18:2 - linoleic acid; 18:3 - linolenic acid; 18:1/18:0 and 18:3/18:2 - ratio of the appropriate fatty acids content; CHOLE - cholesterol; CAMPE - campesterol; SITO - ß-sitosterol; ST - sterols; STIGMA - stigmasterol; DG - digalactosyl diacylgilcerol; MG - monogalactosyl diacylglycerol; LPS - less planar sterols (stigmasterol and sitosterol); MPS - more planar sterols (cholesterol and campesterol); LPS/MPS - ratio of the appropriate sterols content; PL - phospholipids; U/S - ratio of unsaturated to saturated fatty acids; DBI - double bond index.

KW - Fatty acids

KW - Galactolipids

KW - Near-isogenic lines

KW - Phospholipids

KW - Plasmalemma

KW - Rust

KW - Sterols

KW - Wheat

UR - http://www.scopus.com/inward/record.url?scp=2442534374&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442534374&partnerID=8YFLogxK

M3 - Article

VL - 35

SP - 243

EP - 250

JO - Acta Phytopathologica et Entomologica Hungarica

JF - Acta Phytopathologica et Entomologica Hungarica

SN - 0238-1249

IS - 1

ER -