UNTERSCHIEDE FUR IMMUNGLOBULIN E IM SERUM, BESTIMMT MIT KOMPETITIVEM BZW. PAPIER-RADIOIMMUNOSORBENTTEST: BESEITIGUNG DURCH VORBEHANDLUNG MIT POLYETHYLENGLYCOL

Translated title of the contribution: Differences in the levels of serum immunoglobulin E determined by the competitive radio-immuno-sorbent and the paper-radio-immuno-sorbent methods: Removal of these differences by pretreatment of sera with polyethylene glycol

A. Tomcsanyi, B. Fekete, O. Schweiger, L. Kertesz

Research output: Contribution to journalArticle

Abstract

The serum IgE of patients with chronic nonspecific lung diseases was determined with the competitive radioimmuno-sorbent and the paper-radioimmuno-sorbent methods (Phadebas). Some samples showed markedly higher values with the competitive radioimmuno-sorbent than with the paper-radioimmuno-sorbent method. These discrepancies were abolished by pretreatment of the sera: polyethylene glycol 6000 (final concentration 35 g/l) was added, followed by centrifugation at 0°C for 30 min at 20,000 g, and IgE was determined in the supernatant. This treatment had no effect on the values of the paper-radioimmuno-sorbent method, but the values of the competitive radioimmuno-sorbent method were decreased so that the two values agreed within the experimental confidence limits.

Original languageGerman
Pages (from-to)145-146
Number of pages2
JournalJournal of Clinical Chemistry and Clinical Biochemistry
Volume18
Issue number2
Publication statusPublished - Jan 1 1980

    Fingerprint

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this