The serum IgE of patients with chronic nonspecific lung diseases was determined with the competitive radioimmuno-sorbent and the paper-radioimmuno-sorbent methods (Phadebas). Some samples showed markedly higher values with the competitive radioimmuno-sorbent than with the paper-radioimmuno-sorbent method. These discrepancies were abolished by pretreatment of the sera: polyethylene glycol 6000 (final concentration 35 g/l) was added, followed by centrifugation at 0°C for 30 min at 20,000 g, and IgE was determined in the supernatant. This treatment had no effect on the values of the paper-radioimmuno-sorbent method, but the values of the competitive radioimmuno-sorbent method were decreased so that the two values agreed within the experimental confidence limits.
|Number of pages||2|
|Journal||Journal of Clinical Chemistry and Clinical Biochemistry|
|Publication status||Published - Jan 1 1980|
ASJC Scopus subject areas
- Clinical Biochemistry