Differences in the complement activation induced by preformed and nascent immune complexes

T. Hidvegi, L. Varga, A. Falus, M. Kirschfink, M. Kokai, N. A. Tuan, G. Fust

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

While preformed BSA-anti-BSA immune complexes (PIC) bind efficiently to human RBC after their interaction with human complement, nascent BSA-anti-BSA immune complexes (NIC) formed in the presence of complement do not bind to autologous RBC. The same results were obtained with tetanus toxoid-anti-tetanus toxoid PIC and NIC. In order to elucidate the causes of this marked difference between the RBC-binding properties of PIC and NIC, the profile of complement activation induced by them was compared using haemolytic assays and sensitive ELISA tests. BSA-anti-BSA NIC activated C1 more efficiently than PIC. This was reflected in a higher C4 content of the isolated NIC and higher C1 INH-C1s and lower C1q-fibronectin complex level in the NIC-treated serum as compared to the PIC-treated ones. Although isolated NIC contained more C3 than isolated PIC did, there was no significant difference in the AP activation. These findings suggest that the failure of NIC to bind to RBC is not due to a lack of C4-binding, or C3-binding and/or activation, but rather to the special structure of this type of complex.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalComplement and Inflammation
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 1 1991

Keywords

  • Complement activation
  • bovine serum albumin
  • immune complexes
  • tetanus toxoid

ASJC Scopus subject areas

  • Immunology
  • Hematology

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