Differences in Non-MHC Restricted Cytotoxic Activities of Human Peripheral Blood Lymphocytes after Transfusion with Allogeneic Leukocytes or Platelets Possessing Class I and/or Class II MHC Molecules

Éva Pócsik, Rudolf Mihalik, Mariann Réti, Éva Gyódi, Katalin Pálóczi, Klára Mayer, Miklós Kassai, Manfred Herold, Christoph Huber, G. Gyözö Petrányi, Miklós Benczúr

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

MHC-unrestricted cytotoxic activity of peripheral blood lymphocytes (PBL) from 4–6 healthy donors was investigated before and after transfusion with allogeneic leukocytes or platelets. Natural killer and lectin-dependent cellular cytotoxicity (LDCC) of PBL was tested against K562 and Raji target cells in a 4-h and 16-h 51Cr-release assay, respectively. After allotransfusion with leukocytes, we found increased cytotoxic activity of each donor's PBL against all the three targets on day 3 or 7. The highest non-specific cytotoxic activity was detected against the relatively NK resistant Raji target cells. The increase of cytotoxic activity was lowest against the LDCC target (PHA-treated Raji) cells. On the contrary, no changes in cytotoxic activity against any targets were observed after allotransfusion with platelets (possessing class I HLA antigens but no HLA class 11 molecules). Our results suggest that HLA class II molecules, presumably by inducing immune responses, are essential for activation/generation of non-specific killing of tumor targets after leukocyte transfusion. Thrombocytes, known to be less immunogenic than leukocytes, are not effective in in vivo enhancing of non-specific cytotoxicity. Cellular activation of PBL following leukocyte allotransfusion was confirmed by detection of elevated serum neopterin and β-2-micro globulin levels on day 3. This was not the case after platelet allotransfusion. In addition, the expression of ICAM-1 antigen (as a molecule involved directly in MHC-unrestricted cytotoxicity) was also found to be increased in two donors' PBL on day 3 after leukocyte transfusion in contrast to transfusion with platelets.

Original languageEnglish
Pages (from-to)22-31
Number of pages10
JournalImmunobiology
Volume182
Issue number1
DOIs
Publication statusPublished - 1990

Keywords

  • CTL
  • E/T
  • GVHD
  • IFN-γ
  • IL-2 receptor
  • IL-2R
  • LAK
  • LDCC
  • NK
  • PBL
  • T cell receptor
  • TcR
  • WBC
  • cytotoxic T lymphocyte
  • effector to target cell ratio
  • graft-vs.-host disease
  • interferon-γ
  • lectin dependent cellular cytotoxicity
  • lymphokine activated killer (cell)
  • mAb
  • monoclonal antibody
  • natural killer (cell)
  • peripheral blood lymphocytes
  • white blood cells
  • β-2-microglobulin
  • β2-M

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

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