Differences between lymphoid organs with respect to the phosphorylation of deoxycytidine and thymidine

M. Staub, T. Spasokukotskaja, J. Taljanidisz, M. Sasvári, F. Antoni

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The specific activity of thymidine kinase (TK) was higher in spleen than in thymus or unseparated tonsillar lymphocytes, while deoxycytidine kinase (dCK) specific activity was lowest in spleen and was much higher in thymus and in unseparated tonsillar lymphocytes. The ratio of dCK to TK was always high in thymus, in unseparated and in B-cell-enriched tonsillar lymphocytes (between 2 and 5), but it was always low in spleen (0.3-0.4). The difference in the pyrimidine nucleoside phosphorylating enzyme activities of the thymus and spleen does not seem to be a mere consequence of different DNA synthesis rates, because the activities of DNA polymerase-α were practically the same in these organs. Unseparated and B-cell-enriched tonsillar lymphocytes resemble the thymus with respect to the ratio of dCK to TK activities, while the T-cell-enriched fraction contained 3-5 times lower activities of both enzymes. These results suggest that the metabolic pathways of CdR and TdR utilization for DNA synthesis differ in the lymphocyte populations independently from their rate of DNA polymerization and they may be in connection with their maturation processes.

Original languageEnglish
Pages (from-to)137-142
Number of pages6
JournalImmunology Letters
Volume6
Issue number3
DOIs
Publication statusPublished - 1983

Fingerprint

Deoxycytidine
Thymidine
Thymus Gland
Deoxycytidine Kinase
Phosphorylation
Lymphocytes
Thymidine Kinase
Spleen
DNA
B-Lymphocytes
Pyrimidine Nucleosides
DNA-Directed DNA Polymerase
Enzymes
Metabolic Networks and Pathways
Polymerization
T-Lymphocytes
Population

Keywords

  • deoxycytidine kinase
  • lymphocyte maturation
  • thymidine kinase
  • thymus
  • tonsils

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Differences between lymphoid organs with respect to the phosphorylation of deoxycytidine and thymidine. / Staub, M.; Spasokukotskaja, T.; Taljanidisz, J.; Sasvári, M.; Antoni, F.

In: Immunology Letters, Vol. 6, No. 3, 1983, p. 137-142.

Research output: Contribution to journalArticle

Staub, M. ; Spasokukotskaja, T. ; Taljanidisz, J. ; Sasvári, M. ; Antoni, F. / Differences between lymphoid organs with respect to the phosphorylation of deoxycytidine and thymidine. In: Immunology Letters. 1983 ; Vol. 6, No. 3. pp. 137-142.
@article{ab5c497b37244efb9280413e056110ec,
title = "Differences between lymphoid organs with respect to the phosphorylation of deoxycytidine and thymidine",
abstract = "The specific activity of thymidine kinase (TK) was higher in spleen than in thymus or unseparated tonsillar lymphocytes, while deoxycytidine kinase (dCK) specific activity was lowest in spleen and was much higher in thymus and in unseparated tonsillar lymphocytes. The ratio of dCK to TK was always high in thymus, in unseparated and in B-cell-enriched tonsillar lymphocytes (between 2 and 5), but it was always low in spleen (0.3-0.4). The difference in the pyrimidine nucleoside phosphorylating enzyme activities of the thymus and spleen does not seem to be a mere consequence of different DNA synthesis rates, because the activities of DNA polymerase-α were practically the same in these organs. Unseparated and B-cell-enriched tonsillar lymphocytes resemble the thymus with respect to the ratio of dCK to TK activities, while the T-cell-enriched fraction contained 3-5 times lower activities of both enzymes. These results suggest that the metabolic pathways of CdR and TdR utilization for DNA synthesis differ in the lymphocyte populations independently from their rate of DNA polymerization and they may be in connection with their maturation processes.",
keywords = "deoxycytidine kinase, lymphocyte maturation, thymidine kinase, thymus, tonsils",
author = "M. Staub and T. Spasokukotskaja and J. Taljanidisz and M. Sasv{\'a}ri and F. Antoni",
year = "1983",
doi = "10.1016/0165-2478(83)90095-0",
language = "English",
volume = "6",
pages = "137--142",
journal = "Immunology Letters",
issn = "0165-2478",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Differences between lymphoid organs with respect to the phosphorylation of deoxycytidine and thymidine

AU - Staub, M.

AU - Spasokukotskaja, T.

AU - Taljanidisz, J.

AU - Sasvári, M.

AU - Antoni, F.

PY - 1983

Y1 - 1983

N2 - The specific activity of thymidine kinase (TK) was higher in spleen than in thymus or unseparated tonsillar lymphocytes, while deoxycytidine kinase (dCK) specific activity was lowest in spleen and was much higher in thymus and in unseparated tonsillar lymphocytes. The ratio of dCK to TK was always high in thymus, in unseparated and in B-cell-enriched tonsillar lymphocytes (between 2 and 5), but it was always low in spleen (0.3-0.4). The difference in the pyrimidine nucleoside phosphorylating enzyme activities of the thymus and spleen does not seem to be a mere consequence of different DNA synthesis rates, because the activities of DNA polymerase-α were practically the same in these organs. Unseparated and B-cell-enriched tonsillar lymphocytes resemble the thymus with respect to the ratio of dCK to TK activities, while the T-cell-enriched fraction contained 3-5 times lower activities of both enzymes. These results suggest that the metabolic pathways of CdR and TdR utilization for DNA synthesis differ in the lymphocyte populations independently from their rate of DNA polymerization and they may be in connection with their maturation processes.

AB - The specific activity of thymidine kinase (TK) was higher in spleen than in thymus or unseparated tonsillar lymphocytes, while deoxycytidine kinase (dCK) specific activity was lowest in spleen and was much higher in thymus and in unseparated tonsillar lymphocytes. The ratio of dCK to TK was always high in thymus, in unseparated and in B-cell-enriched tonsillar lymphocytes (between 2 and 5), but it was always low in spleen (0.3-0.4). The difference in the pyrimidine nucleoside phosphorylating enzyme activities of the thymus and spleen does not seem to be a mere consequence of different DNA synthesis rates, because the activities of DNA polymerase-α were practically the same in these organs. Unseparated and B-cell-enriched tonsillar lymphocytes resemble the thymus with respect to the ratio of dCK to TK activities, while the T-cell-enriched fraction contained 3-5 times lower activities of both enzymes. These results suggest that the metabolic pathways of CdR and TdR utilization for DNA synthesis differ in the lymphocyte populations independently from their rate of DNA polymerization and they may be in connection with their maturation processes.

KW - deoxycytidine kinase

KW - lymphocyte maturation

KW - thymidine kinase

KW - thymus

KW - tonsils

UR - http://www.scopus.com/inward/record.url?scp=0020585056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020585056&partnerID=8YFLogxK

U2 - 10.1016/0165-2478(83)90095-0

DO - 10.1016/0165-2478(83)90095-0

M3 - Article

VL - 6

SP - 137

EP - 142

JO - Immunology Letters

JF - Immunology Letters

SN - 0165-2478

IS - 3

ER -