Difference in the production of human interferon-α and -β in mouse cells

M. I. Toth, O. Cendsuren, V. Endresz, I. Karcagi, E. Duda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Human inteferon-α1 and interferon-β genes with their flanking regions were introduced into mouse LMTK- cells. Although transfected cells contained the interferon genes with a similar copy number and produced a similar amount of interferon-specific mRNA, cells containing the human interferon-β gene secreted about 10 times more human interferon than cells transfected with the human interferon-α1 gene. When the coding region of the interferon-β gene was replaced by that of the interferon-α1 gene (hybrid interferon β/α gene), the human interferon production of transfected cells fell by approx. one order of magnitude. These results show that in the case of exogenous interferon genes a translational or post-translational mechanism might significantly affect the final level of human interferons, resulting in higher titres of interferon-β than of interferon-α.

Original languageEnglish
Pages (from-to)2527-2533
Number of pages7
JournalJournal of General Virology
Volume69
Issue number10
DOIs
Publication statusPublished - Jan 1 1988

ASJC Scopus subject areas

  • Virology

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