Diethylnitrosamine induces lung adenocarcinoma in FVB/N mouse

Zsolt Mervai, Krisztina Egedi, I. Kovalszky, Kornélia Baghy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Diethylnitrosamine is a well known carcinogen that induces cancers of various organs in mice and rats. Using FVB/N mouse strain, here we show that diethylnitrosamine induces primarily lung adenocarcinomas with modest tumor development in the liver, offering a new model to study chemical carcinogenesis in the lung. Methods: Animals were exposed to a single high dose of diethylnitrosamine, and more than 70% of the mice developed lung cancer. To obtain a new transplantable tumor line, pieces of primary tumors were inoculated and maintained subcutaneously in the same mouse strain. We used immunohistochemistry to characterize the tumor for main lung adenocarcinoma markers. We searched for mutations in KRAS exon 2 and EGFR exon 19, 21 with Sanger sequencing. We also compared the normal lung tissue with the diethylnitrosamine induced primary adenocarcinoma, and with the subcutaneously maintained adenocarcinoma using Western blot technique for main cell cycle markers and to identify the main pathways. Results: Primary and subcutaneous tumors express cytokeratin-7 and thyroid transcription factor-1, markers characteristic to lung adenocarcinoma. In addition, no mutations were found in the hot spot regions of KRAS and EGFR genes. We found high mTOR activation, but the level of p-Akt Ser473 and p-Akt Thr308 decreased in the tumorous samples. Conclusions: We established a new lung adenocarcinoma model using FVB/N mouse strain and diethylnitrosamine. We believe that this new model system would be highly useful in lung cancer research.

Original languageEnglish
Article number157
JournalBMC Cancer
Volume18
Issue number1
DOIs
Publication statusPublished - Feb 7 2018

Fingerprint

Diethylnitrosamine
Neoplasms
Exons
Lung Neoplasms
Adenocarcinoma
Keratin-7
erbB-1 Genes
Lung
Mutation
Carcinogens
Adenocarcinoma of lung
Cell Cycle
Carcinogenesis
Western Blotting
Immunohistochemistry
Liver
Research

Keywords

  • Diethylnitrosamine
  • Lung cancer
  • Mouse model
  • NSCLC
  • Tumorigenesis

ASJC Scopus subject areas

  • Oncology
  • Genetics
  • Cancer Research

Cite this

Diethylnitrosamine induces lung adenocarcinoma in FVB/N mouse. / Mervai, Zsolt; Egedi, Krisztina; Kovalszky, I.; Baghy, Kornélia.

In: BMC Cancer, Vol. 18, No. 1, 157, 07.02.2018.

Research output: Contribution to journalArticle

Mervai, Zsolt ; Egedi, Krisztina ; Kovalszky, I. ; Baghy, Kornélia. / Diethylnitrosamine induces lung adenocarcinoma in FVB/N mouse. In: BMC Cancer. 2018 ; Vol. 18, No. 1.
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