Diet-induced obesity skin changes monitored by in vivo SHG and ex vivo CARS microscopy

Dóra Haluszka, Kende Lőrincz, Norbert Kiss, R. Szipócs, Enikő Kuroli, Nóra Gyöngyösi, Norbert M. Wikonkál

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Obesity related metabolic syndrome and type 2 diabetes have severe consequences on our skin. Latest developments in nonlinear microscopy allow the use of noninvasive, label free imaging methods, such as second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS), for early diagnosis of metabolic syndrome-related skin complications by 3D imaging of the skin and the connective tissue. Our aim was to study effects of various types of diet-induced obesity in mice using these methods. We examined mice on different diets for 32 weeks. The collagen morphology was evaluated four times in vivo by SHG microscopy, and adipocytes were examined once at the end of experiment by ex vivo CARS method. A strong correlation was found between the body weight and the adipocyte size, while we found that the SHG intensity of dermal collagen reduces considerably with increasing body weight. Obese mice on high-fat diet showed worse results than those on high-fat-high-fructose diet. Animals on high-fructose diet did not gain more weight than those on ordinary diet despite of the increased calorie intake, but their collagen damage was nonetheless significant. Obesity and high sugar intake damages the skin, mainly the dermal connective tissue and subcutaneous adipose tissue, which efficiently can be monitored by in vivo SHG and ex vivo CARS microscopy.

Original languageEnglish
Article number#268324
Pages (from-to)4480-4489
Number of pages10
JournalBiomedical Optics Express
Volume7
Issue number11
DOIs
Publication statusPublished - Nov 1 2016

Fingerprint

obesity
diets
Raman Spectrum Analysis
Microscopy
harmonic generations
Obesity
Raman spectra
Diet
microscopy
Skin
collagens
mice
connective tissue
body weight
Collagen
fats
High Fat Diet
Fructose
Adipocytes
Connective Tissue

Keywords

  • Dermatology
  • Nonlinear microscopy

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Haluszka, D., Lőrincz, K., Kiss, N., Szipócs, R., Kuroli, E., Gyöngyösi, N., & Wikonkál, N. M. (2016). Diet-induced obesity skin changes monitored by in vivo SHG and ex vivo CARS microscopy. Biomedical Optics Express, 7(11), 4480-4489. [#268324]. https://doi.org/10.1364/BOE.7.004480

Diet-induced obesity skin changes monitored by in vivo SHG and ex vivo CARS microscopy. / Haluszka, Dóra; Lőrincz, Kende; Kiss, Norbert; Szipócs, R.; Kuroli, Enikő; Gyöngyösi, Nóra; Wikonkál, Norbert M.

In: Biomedical Optics Express, Vol. 7, No. 11, #268324, 01.11.2016, p. 4480-4489.

Research output: Contribution to journalArticle

Haluszka, D, Lőrincz, K, Kiss, N, Szipócs, R, Kuroli, E, Gyöngyösi, N & Wikonkál, NM 2016, 'Diet-induced obesity skin changes monitored by in vivo SHG and ex vivo CARS microscopy', Biomedical Optics Express, vol. 7, no. 11, #268324, pp. 4480-4489. https://doi.org/10.1364/BOE.7.004480
Haluszka, Dóra ; Lőrincz, Kende ; Kiss, Norbert ; Szipócs, R. ; Kuroli, Enikő ; Gyöngyösi, Nóra ; Wikonkál, Norbert M. / Diet-induced obesity skin changes monitored by in vivo SHG and ex vivo CARS microscopy. In: Biomedical Optics Express. 2016 ; Vol. 7, No. 11. pp. 4480-4489.
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