Juhok és kecskék fertozo elapasztásának megállapítása Magyarországon

Translated title of the contribution: Diagnostics of contagious agalactia in Hungary

Bajmócy Endre, Kaszanyitzky Éva, Göran Bölske, Matiz Katalin, Tanyi János

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Abstract

Contagious agalactia is not endemic in Hungary. Apart from the occurrence reported here, there has been only one outbreak of the disease 50 years ago when it was successfully eradicated by stamping out. In the summer of 1997 there was also a mass outbreak on one farm among 200 mother goats and 500 ewes where in the course of one month about 150 animals got ill. The sick animals developed keratoconjunctivitis (Fig. 1) often leading to blindness, polyarthritis that caused lameness and downer syndrome (Fig. 2 and 3), and interstitial mastitis that led to atrophy of the udder. The clinical symptoms were suggestive of a disease of mycoplasmal origin. Other bacterial, chlamydial, and viral aetiological agents have been eliminated from the differential diagnostic possibilities by routine diagnostic methods. The presence of Mycoplasma in synovial fluid of the ill animals (Fig. 4) was confirmed by PCR (Fig. 5). Mycoplasma were cultured from the synovial, tear and milk samples taken from a total of 14 animals. Based on their biochemical characteristics and the results of growth inhibition test with positive sera (Table 2) the isolates were identified as Mycoplasma agalactiae. After sequencing, the 270 base-pair long PCR product gained from the Mycoplasma monoculture proved to be 100% identical with the corresponding gene sequence of M.agalactiae coding the 16S ribosomal RNA. Consequently the diagnosis of contagious agalactia was established and the affected herd stamped out.

Original languageHungarian
Pages (from-to)390-394
Number of pages5
JournalMagyar Allatorvosok Lapja
Volume120
Issue number7
Publication statusPublished - Dec 1 1998

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ASJC Scopus subject areas

  • veterinary(all)

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