Kutya orsósejtes haemangiomájának diagnózisa klaudin-5 marker segítségével: Esetismertetes

Translated title of the contribution: Diagnosis of spindle cell haemangioma in a dog by the use of claudin-5. Case report

Jakab Csaba, Balka Gyula, Rusvai Miklos, Gálfi Péter, Stark Róbert, Kulka Janina

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Abstract

The authors diagnosed a rare type of canine cutaneous haemangioma, spindle cell haemangioma (SCH) in a surgically removed tissue specimen obtained from the right hind limb of a 6 years old German Shepard. The examination of the slides stained with haematoxylin and eosin revealed that 80% of the sample consisted of solid proliferation of differentiated, elongated, spindle-like monomorphic mesenchymal cells {Figure 1 and 2). The cells resembling fibroblasts had hyperchromatic spindle shaped nuclei (Figure 3). No dividing cells were observed. In the smaller part of the sample erythrocytes containing partially collapsed vascular proliferates were observed, that were lined by one layer of endothelial cells The immunohistological analysis of the slides revealed, that approximately 60-70% of the solid, spindle cell population showed intense immunoreactivity with claudin-5 {Figure 4 and 5) and CD31, as well as the one cell layered endothel lining of the collapsed, neoplastic blood vessels. The tumour cells showed more intense claudin-5 positivity compared to the weaker but still unequivocal CD31 reaction. Negative immunoreactivity was obtained with the endothel markers (claudin-5, CD31) in case of 30-40% of the spindle shaped, other mesenchymal cells (non-endothelial cells, fibroblasts). Every spindle cell, however gave intense positive reaction for vimentin (Figure 6), but negative reaction for α-SMA. For the alpha;-SMA investigations, smooth muscle cells in the tunica media of the normal arteries were used as internal positive controls (Figure 7). The tumour cells did not show NSE and melan-A immunoreactivity. During the routine examination of the histological specimens benign cutaneous spindle cell tumours, such as dermatofibroma, haemangiopericytoma, myopericytoma, angioleiomyoma, piloleio-myoma, benign periferial nerve sheath tumour, dermal spindle cell melanocytoma, lymph-angioma, non-neoplastic nodular dermatofibrosis, and granulation tissue were considered in the differential diagnosis of the spindle cell haemangioma. Because the differentiation was not possible, based on the analysis of the slides stained with HE, immuno-histochemistry staining carried out on the sections to detect claudin-5, CD31 as endothelial markers, vimentin (mesenchymal marker), alpha;-SMA (muscle marker), NSE (neural tissue marker) and melan-A (melanocyte marker) led to the final diagnosis. For the diagnosis of the rarely occurring canine SCH claudin-5 molecule was used for the first time in veterinary tumour pathology.

Translated title of the contributionDiagnosis of spindle cell haemangioma in a dog by the use of claudin-5. Case report
Original languageHungarian
Pages (from-to)402-406
Number of pages5
JournalMagyar Allatorvosok Lapja
Volume131
Issue number7
Publication statusPublished - Jul 1 2009

ASJC Scopus subject areas

  • veterinary(all)

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