Dextran-based hydrogel layers for biosensors

Andras Saftics, Barbara Türk, A. Sulyok, Norbert Nagy, Emil Agócs, Benjámin Kalas, Péter Petrik, Miklós Fried, Nguyen Quoc Khánh, Aurél Prósz, K. Kamarás, Inna Szekacs, Robert Horvath, Sándor Kurunczi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Biofunctional coatings are key elements of biosensors regulating interactions between the sensing surface and analytes as well as matrix components of the sample. These coatings can improve sensing capabilities both by amplifying the target signal and attenuating interfering signals originating from surface fouling (non-specific binding). Considering the tested materials so far, hydrogel-based layers have been verified to be among the most effective layers in improving biochip performance. The polysaccharide dextran can be efficiently used to form hydrogel layers displaying extended three-dimensional structure on biosensor surfaces. Owing to their high water content and flexible structure, dextran coatings present advanced antifouling abilities, which can be exploited in classic bioanalytical measurements as well as in the development of cell-on-a-chip type biosensors. However, in spite of the numerous applications, the deep characterization of dextran layers has been missing from the literature. This phenomenon can be attributed to the challenging analysis of few nanometer-thick layers with high water content. The lack of available data is more pronounced regarding the layer behaviors under aqueous conditions. In this chapter we present various surface analytical methods (including biosensor-type techniques) suitable for the complex characterization of hydrogel coatings whose thickness ranges from few to several ten nanometers. As a case study, we focus on the analysis of carboxymethyl dextran (CMD) layers developed for waveguidebased label-free optical biosensor applications. Examination methodologies both under dry and aqueous conditions as well as testing of antifouling abilities are also presented.

Original languageEnglish
Title of host publicationNanobiomaterial Engineering
Subtitle of host publicationConcepts and Their Applications in Biomedicine and Diagnostics
PublisherSpringer Singapore
Pages139-164
Number of pages26
ISBN (Electronic)9789813298408
ISBN (Print)9789813298392
DOIs
Publication statusPublished - Jan 1 2020

Keywords

  • Dextran
  • Ellipsometry
  • Hydrogel
  • Label-free biosensor
  • Non-specific binding
  • OWLS
  • QCM

ASJC Scopus subject areas

  • Medicine(all)
  • Health Professions(all)
  • Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Saftics, A., Türk, B., Sulyok, A., Nagy, N., Agócs, E., Kalas, B., Petrik, P., Fried, M., Khánh, N. Q., Prósz, A., Kamarás, K., Szekacs, I., Horvath, R., & Kurunczi, S. (2020). Dextran-based hydrogel layers for biosensors. In Nanobiomaterial Engineering: Concepts and Their Applications in Biomedicine and Diagnostics (pp. 139-164). Springer Singapore. https://doi.org/10.1007/978-981-32-9840-8_8