Dextran-based hydrogel formed by thiol-Michael addition reaction for 3D cell encapsulation

Zhen Qi Liu, Zhao Wei, Xv Long Zhu, Guo You Huang, Feng Xu, Jian Hai Yang, Yoshihito Osada, Miklós Zrínyi, Jian Hui Li, Yong Mei Chen

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Cell encapsulation in three-dimensional (3D) hydrogels can mimic native cell microenvironment and plays a major role in cell-based transplantation therapies. In this contribution, a novel in situ-forming hydrogel, Dex-l-DTT hydrogel ("l" means "linked-by"), by cross-linking glycidyl methacrylate derivatized dextran (Dex-GMA) and dithiothreitol (DTT) under physiological conditions, has been developed using thiol-Michael addition reaction. The mechanical properties, gelation process and degree of swelling of the hydrogel can be easily adjusted by changing the pH of phosphate buffer saline. The 3D cell encapsulation ability is demonstrated by encapsulating rat bone marrow mesenchymal stem cells (BMSCs) and NIH/3T3 fibroblasts into the in situ-forming hydrogel with maintained high viability. The BMSCs also maintain their differentiation potential after encapsulation. These results demonstrate that the Dex-l-DTT hydrogel holds great potential for biomedical field.

Original languageEnglish
Pages (from-to)140-148
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume128
DOIs
Publication statusPublished - Apr 1 2015

Keywords

  • 3D cell encapsulation
  • Bone marrow mesenchymal stem cells
  • Dextran
  • Hydrogel
  • Thiol-Michael addition

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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  • Cite this

    Liu, Z. Q., Wei, Z., Zhu, X. L., Huang, G. Y., Xu, F., Yang, J. H., Osada, Y., Zrínyi, M., Li, J. H., & Chen, Y. M. (2015). Dextran-based hydrogel formed by thiol-Michael addition reaction for 3D cell encapsulation. Colloids and Surfaces B: Biointerfaces, 128, 140-148. https://doi.org/10.1016/j.colsurfb.2015.02.005