Deviations in circulating TNF levels and TNF production by mononuclear cells in healthy human populations

T. Mózes, I. Baráth, K. Gornicsar, A. Grosz, Ta Mózes, Cs Göndöcs, P. Széphalmi, K. Gaál, E. Madarász

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objectives. Tumor necrosis factor alpha (TNF ) plays a pivotal role in the inflammatory host response. The serum-level of TNF and the production of TNF by lympho/monocytes, however, seem to show high individual variations. The goal of the present study was to investigate the variations and inducibility of TNF -activity in two age-groups of healthy volunteers. Methods. Sixty elderly, healthy volunteers were studied. These persons were free of malignant diseases, and within three months, they did not have any trauma or inflammatory disease and were not taking any steroids or nonsteroid anti-inflammatory drugs. Thirty young volunteers were also included. Blood samples were taken; lympho/monocytes were separated and cultured with or without endotoxin (LPS) stimulation. Serum and culture supernatant TNF levels were determined by bioassay using WEHI 164 cells. Results. The results indicated significant individual variations in TNF levels of healthy volunteers irrespective of age. Subgroups with low, middle, and high serum TNF-levels were distinguished. In about 50 of volunteers with low serum-TNF activity, LPS stimulation failed to increase the TNF production by isolated lympho/monocytes. Conclusion. Our data suggest a chance to select individuals with enhanced sensitivity for septic complications.

Original languageEnglish
Article number972609
JournalMediators of Inflammation
Volume2011
DOIs
Publication statusPublished - 2011

Fingerprint

Tumor Necrosis Factor-alpha
Population
Monocytes
Healthy Volunteers
Serum
Volunteers
Endotoxins
Biological Assay
Anti-Inflammatory Agents
Age Groups
Steroids
Wounds and Injuries
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Deviations in circulating TNF levels and TNF production by mononuclear cells in healthy human populations. / Mózes, T.; Baráth, I.; Gornicsar, K.; Grosz, A.; Mózes, Ta; Göndöcs, Cs; Széphalmi, P.; Gaál, K.; Madarász, E.

In: Mediators of Inflammation, Vol. 2011, 972609, 2011.

Research output: Contribution to journalArticle

Mózes, T, Baráth, I, Gornicsar, K, Grosz, A, Mózes, T, Göndöcs, C, Széphalmi, P, Gaál, K & Madarász, E 2011, 'Deviations in circulating TNF levels and TNF production by mononuclear cells in healthy human populations', Mediators of Inflammation, vol. 2011, 972609. https://doi.org/10.1155/2011/972609
Mózes, T. ; Baráth, I. ; Gornicsar, K. ; Grosz, A. ; Mózes, Ta ; Göndöcs, Cs ; Széphalmi, P. ; Gaál, K. ; Madarász, E. / Deviations in circulating TNF levels and TNF production by mononuclear cells in healthy human populations. In: Mediators of Inflammation. 2011 ; Vol. 2011.
@article{bccaf63980c4419daaac9b21f75de691,
title = "Deviations in circulating TNF levels and TNF production by mononuclear cells in healthy human populations",
abstract = "Objectives. Tumor necrosis factor alpha (TNF ) plays a pivotal role in the inflammatory host response. The serum-level of TNF and the production of TNF by lympho/monocytes, however, seem to show high individual variations. The goal of the present study was to investigate the variations and inducibility of TNF -activity in two age-groups of healthy volunteers. Methods. Sixty elderly, healthy volunteers were studied. These persons were free of malignant diseases, and within three months, they did not have any trauma or inflammatory disease and were not taking any steroids or nonsteroid anti-inflammatory drugs. Thirty young volunteers were also included. Blood samples were taken; lympho/monocytes were separated and cultured with or without endotoxin (LPS) stimulation. Serum and culture supernatant TNF levels were determined by bioassay using WEHI 164 cells. Results. The results indicated significant individual variations in TNF levels of healthy volunteers irrespective of age. Subgroups with low, middle, and high serum TNF-levels were distinguished. In about 50 of volunteers with low serum-TNF activity, LPS stimulation failed to increase the TNF production by isolated lympho/monocytes. Conclusion. Our data suggest a chance to select individuals with enhanced sensitivity for septic complications.",
author = "T. M{\'o}zes and I. Bar{\'a}th and K. Gornicsar and A. Grosz and Ta M{\'o}zes and Cs G{\"o}nd{\"o}cs and P. Sz{\'e}phalmi and K. Ga{\'a}l and E. Madar{\'a}sz",
year = "2011",
doi = "10.1155/2011/972609",
language = "English",
volume = "2011",
journal = "Mediators of Inflammation",
issn = "0962-9351",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Deviations in circulating TNF levels and TNF production by mononuclear cells in healthy human populations

AU - Mózes, T.

AU - Baráth, I.

AU - Gornicsar, K.

AU - Grosz, A.

AU - Mózes, Ta

AU - Göndöcs, Cs

AU - Széphalmi, P.

AU - Gaál, K.

AU - Madarász, E.

PY - 2011

Y1 - 2011

N2 - Objectives. Tumor necrosis factor alpha (TNF ) plays a pivotal role in the inflammatory host response. The serum-level of TNF and the production of TNF by lympho/monocytes, however, seem to show high individual variations. The goal of the present study was to investigate the variations and inducibility of TNF -activity in two age-groups of healthy volunteers. Methods. Sixty elderly, healthy volunteers were studied. These persons were free of malignant diseases, and within three months, they did not have any trauma or inflammatory disease and were not taking any steroids or nonsteroid anti-inflammatory drugs. Thirty young volunteers were also included. Blood samples were taken; lympho/monocytes were separated and cultured with or without endotoxin (LPS) stimulation. Serum and culture supernatant TNF levels were determined by bioassay using WEHI 164 cells. Results. The results indicated significant individual variations in TNF levels of healthy volunteers irrespective of age. Subgroups with low, middle, and high serum TNF-levels were distinguished. In about 50 of volunteers with low serum-TNF activity, LPS stimulation failed to increase the TNF production by isolated lympho/monocytes. Conclusion. Our data suggest a chance to select individuals with enhanced sensitivity for septic complications.

AB - Objectives. Tumor necrosis factor alpha (TNF ) plays a pivotal role in the inflammatory host response. The serum-level of TNF and the production of TNF by lympho/monocytes, however, seem to show high individual variations. The goal of the present study was to investigate the variations and inducibility of TNF -activity in two age-groups of healthy volunteers. Methods. Sixty elderly, healthy volunteers were studied. These persons were free of malignant diseases, and within three months, they did not have any trauma or inflammatory disease and were not taking any steroids or nonsteroid anti-inflammatory drugs. Thirty young volunteers were also included. Blood samples were taken; lympho/monocytes were separated and cultured with or without endotoxin (LPS) stimulation. Serum and culture supernatant TNF levels were determined by bioassay using WEHI 164 cells. Results. The results indicated significant individual variations in TNF levels of healthy volunteers irrespective of age. Subgroups with low, middle, and high serum TNF-levels were distinguished. In about 50 of volunteers with low serum-TNF activity, LPS stimulation failed to increase the TNF production by isolated lympho/monocytes. Conclusion. Our data suggest a chance to select individuals with enhanced sensitivity for septic complications.

UR - http://www.scopus.com/inward/record.url?scp=84860219106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860219106&partnerID=8YFLogxK

U2 - 10.1155/2011/972609

DO - 10.1155/2011/972609

M3 - Article

C2 - 21860543

AN - SCOPUS:84860219106

VL - 2011

JO - Mediators of Inflammation

JF - Mediators of Inflammation

SN - 0962-9351

M1 - 972609

ER -