Developmental regulation of dUTPase in Drosophila melanogaster

Angéla Békési, Imre Zagyva, E. Hunyadi-Gulyás, Veronika Pongrácz, Júlia Kovári, Ágnes O. Nagy, A. Erdei, Katalin F. Medzihradszky, B. Vértessy

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

dUTPase prevents uracil incorporation into DNA by strict regulation of the cellular dUTP:dTTP ratio. Lack of the enzyme initiates thymineless cell death, prompting studies on enzyme regulation. We investigated expression pattern and localization of Drosophila dUTPase. Similarly to human, two isoforms of the fly enzyme were identified at both mRNA and protein levels. During larval stages, a drastic decrease of dUTPase expression was demonstrated at the protein level. In contrast, dUTPase mRNAs display constitutive character throughout development. A putative nuclear localization signal was identified in one of the two isoforms. However, immunohistochemistry of ovaries and embryos did not show a clear correlation between the presence of this signal and subcellular localization of the protein, suggesting that the latter may be perturbed by additional factors. Results are in agreement with a multilevel regulation of dUTPase in the Drosophila proteome, possibly involving several interacting protein partners of the enzyme. Using independent approaches, the existence of such macromolecular partners was verified.

Original languageEnglish
Pages (from-to)22362-22370
Number of pages9
JournalJournal of Biological Chemistry
Volume279
Issue number21
DOIs
Publication statusPublished - May 21 2004

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Drosophila melanogaster
Enzymes
Drosophila
Protein Isoforms
Proteins
Nuclear Localization Signals
Messenger RNA
Uracil
Cell death
Proteome
Diptera
Ovary
Cell Death
Embryonic Structures
Immunohistochemistry
dUTP pyrophosphatase
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Developmental regulation of dUTPase in Drosophila melanogaster. / Békési, Angéla; Zagyva, Imre; Hunyadi-Gulyás, E.; Pongrácz, Veronika; Kovári, Júlia; Nagy, Ágnes O.; Erdei, A.; Medzihradszky, Katalin F.; Vértessy, B.

In: Journal of Biological Chemistry, Vol. 279, No. 21, 21.05.2004, p. 22362-22370.

Research output: Contribution to journalArticle

Békési, A, Zagyva, I, Hunyadi-Gulyás, E, Pongrácz, V, Kovári, J, Nagy, ÁO, Erdei, A, Medzihradszky, KF & Vértessy, B 2004, 'Developmental regulation of dUTPase in Drosophila melanogaster', Journal of Biological Chemistry, vol. 279, no. 21, pp. 22362-22370. https://doi.org/10.1074/jbc.M313647200
Békési, Angéla ; Zagyva, Imre ; Hunyadi-Gulyás, E. ; Pongrácz, Veronika ; Kovári, Júlia ; Nagy, Ágnes O. ; Erdei, A. ; Medzihradszky, Katalin F. ; Vértessy, B. / Developmental regulation of dUTPase in Drosophila melanogaster. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 21. pp. 22362-22370.
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