Developmental differences in B cell receptor-induced signal transduction

Dorottya Kövesdi, Gábor Koncz, Roland Iványi-Nagy, Yael Caspi, Masamichi Ishiai, Tomohiro Kurosaki, János Gergely, Joseph Haimovich, Gabriella Sármay

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have compared early signaling events at various stages of B cell differentiation using established mouse cell lines. Clustering of pre-B cell antigen receptor (BCR) or BCR induced the tyrosine phosphorylation of various proteins in all cells, although the phosphorylation pattern differed. In spite of the pre-BCR-induced tyrosine phosphorylation, we could not detect an intracellular Ca2+ signal in pre-B cells. However, coclustering of the pre-BCR with CD19 did induce Ca2+ mobilization. In contrast to the immature and mature B cells, where the B cell linker protein (BLNK) went through inducible tyrosine phosphorylation upon BCR clustering, we observed a constitutive tyrosine phosphorylation of BLNK in pre-B cell lines. Both BLNK and phospholipase C (PLC)γ were raft associated in unstimulated pre-B cells, and this could not be enhanced by pre-BCR engagement, suggesting a ligand-independent PLCγ-mediated signaling. Further results indicate that the cell lines representing the immature stage are more sensitive to BCR-, CD19- and type II receptors binding the Fc part of IgG (FcγRIIb)-mediated signals than mature B cells.

Original languageEnglish
Pages (from-to)563-572
Number of pages10
JournalCellular Signalling
Volume14
Issue number6
DOIs
Publication statusPublished - Mar 26 2002

Keywords

  • B cell
  • Development
  • Receptors
  • Signaling
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Cell Biology

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