Developmental differences in B cell receptor-induced signal transduction

Dorottya Kövesdi, G. Koncz, Roland Iványi-Nagy, Yael Caspi, Masamichi Ishiai, Tomohiro Kurosaki, J. Gergely, Joseph Haimovich, G. Sármay

Research output: Contribution to journalArticle

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Abstract

We have compared early signaling events at various stages of B cell differentiation using established mouse cell lines. Clustering of pre-B cell antigen receptor (BCR) or BCR induced the tyrosine phosphorylation of various proteins in all cells, although the phosphorylation pattern differed. In spite of the pre-BCR-induced tyrosine phosphorylation, we could not detect an intracellular Ca2+ signal in pre-B cells. However, coclustering of the pre-BCR with CD19 did induce Ca2+ mobilization. In contrast to the immature and mature B cells, where the B cell linker protein (BLNK) went through inducible tyrosine phosphorylation upon BCR clustering, we observed a constitutive tyrosine phosphorylation of BLNK in pre-B cell lines. Both BLNK and phospholipase C (PLC)γ were raft associated in unstimulated pre-B cells, and this could not be enhanced by pre-BCR engagement, suggesting a ligand-independent PLCγ-mediated signaling. Further results indicate that the cell lines representing the immature stage are more sensitive to BCR-, CD19- and type II receptors binding the Fc part of IgG (FcγRIIb)-mediated signals than mature B cells.

Original languageEnglish
Pages (from-to)563-572
Number of pages10
JournalCellular Signalling
Volume14
Issue number6
DOIs
Publication statusPublished - 2002

Fingerprint

B-Cell Antigen Receptors
Pre-B Cell Receptors
Signal Transduction
B-Lymphocytes
B-Lymphoid Precursor Cells
Phosphorylation
Tyrosine
Type C Phospholipases
Cell Line
Cluster Analysis
CD19 Antigens
Fc Receptors
Cell Differentiation
Immunoglobulin G
Ligands

Keywords

  • B cell
  • Development
  • Receptors
  • Signaling
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Developmental differences in B cell receptor-induced signal transduction. / Kövesdi, Dorottya; Koncz, G.; Iványi-Nagy, Roland; Caspi, Yael; Ishiai, Masamichi; Kurosaki, Tomohiro; Gergely, J.; Haimovich, Joseph; Sármay, G.

In: Cellular Signalling, Vol. 14, No. 6, 2002, p. 563-572.

Research output: Contribution to journalArticle

Kövesdi, D, Koncz, G, Iványi-Nagy, R, Caspi, Y, Ishiai, M, Kurosaki, T, Gergely, J, Haimovich, J & Sármay, G 2002, 'Developmental differences in B cell receptor-induced signal transduction', Cellular Signalling, vol. 14, no. 6, pp. 563-572. https://doi.org/10.1016/S0898-6568(01)00274-1
Kövesdi, Dorottya ; Koncz, G. ; Iványi-Nagy, Roland ; Caspi, Yael ; Ishiai, Masamichi ; Kurosaki, Tomohiro ; Gergely, J. ; Haimovich, Joseph ; Sármay, G. / Developmental differences in B cell receptor-induced signal transduction. In: Cellular Signalling. 2002 ; Vol. 14, No. 6. pp. 563-572.
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