Developmental analysis of primary and secondary somatic embryogenesis in soybean tissue culture.

G. Gyulai, E. Kiss, A. Csillag, L. E. Heszky

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Somatic embryogenesis from immature soybean cotyledons was induced on Murashige & Skoog medium, supplemented with 2,4-dichlorophenoxyacetic acid. The full developmental analyses of primary and secondary somatic embryogenesis was monitored by scanning electron microscopy. The developmental stages of embryogenesis from the first unequal cell division through the three- and four-cell embryos, and multi-cell globular and heart shape embryos, to the fully developed plants were determined. When the globular stage embryos were incubated on the embryo maturation medium for 4 to 6 weeks a secondary embryogenesis was observed and scanned. Chimeric embryos with epidermis zones similar to mature leaf were characterized.

Original languageEnglish
Pages (from-to)189-196
Number of pages8
JournalActa biologica Hungarica
Volume44
Issue number2-3
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

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