Development to the blastocyst stage of parthenogenetically activated in vitro matured porcine oocytes after solid surface vitrification (SSV)

Tamás Somfai, A. Dinnyés, Dagmar Sage, Miklós Marosán, Joseph W. Carnwath, Manabu Ozawa, Kazuhiro Kikuchi, Heiner Niemann

Research output: Contribution to journalArticle

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Abstract

The effects of solid surface vitrification (SSV) on viability and parthenogenetic development of in vitro matured (IVM) porcine oocytes was investigated in the present study. Cumulus-free IVM porcine oocytes were subjected either to SSV or SSV combined with a cytochalasin B (CB) pre-treatment (SSV + CB) or all steps of SSV but without cooling (toxicity control = TC; toxicity control with CB pre-treatment = TC + CB). Oocyte viability was evaluated by plasma membrane integrity and esterase activity measured by a combined staining with fluorescein diacetate, propidium iodide and Hoechst 33342. Surviving oocytes were parthenogenetically activated then cultured in vitro (IVC) for 6 days. The proportion of live oocytes after vitrification was significantly lower than that of the TC, TC + CB and the control groups, regardless of the CB pre-treatment. Treatment of oocytes with cryoprotectants did not decrease the rates of surviving oocytes. After activation of oocytes, the proportion of cleaved embryos was significantly higher in the SSV + CB (P <0.05) than that of the SSV group. Nevertheless, significantly more oocytes cleaved (P <0.05) in the TC, TC + CB and the control groups. On Day 6, the rate of blastocysts in the SSV and SSV + CB groups did not differ significantly. The number of oocytes developing to blastocyst and the mean number of blastomeres per embryo were significantly higher (P <0.05) in the TC, TC + CB and the control compared with that of the SSV and SSV + CB groups. To our knowledge, this is the first report on parthenogenetic development to blastocysts of porcine oocytes vitrified at the metaphase-II stage. Results indicate that the high concentrations of cryoprotectants were not harmful for in vitro development, and that CB pre-treatment may increase survival and development of SSV vitrified porcine oocytes.

Original languageEnglish
Pages (from-to)415-422
Number of pages8
JournalTheriogenology
Volume66
Issue number2
DOIs
Publication statusPublished - Jul 15 2006

Fingerprint

Vitrification
cytochalasin B
Cytochalasin B
vitrification
Blastocyst
blastocyst
Oocytes
oocytes
Swine
swine
pretreatment
cryoprotectants
In Vitro Techniques
embryo (animal)
Embryonic Structures
viability
toxicity
Blastomeres
Control Groups
blastomeres

Keywords

  • Blastocyst
  • IVM
  • Oocyte
  • Porcine
  • Vitrification

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Development to the blastocyst stage of parthenogenetically activated in vitro matured porcine oocytes after solid surface vitrification (SSV). / Somfai, Tamás; Dinnyés, A.; Sage, Dagmar; Marosán, Miklós; Carnwath, Joseph W.; Ozawa, Manabu; Kikuchi, Kazuhiro; Niemann, Heiner.

In: Theriogenology, Vol. 66, No. 2, 15.07.2006, p. 415-422.

Research output: Contribution to journalArticle

Somfai, Tamás ; Dinnyés, A. ; Sage, Dagmar ; Marosán, Miklós ; Carnwath, Joseph W. ; Ozawa, Manabu ; Kikuchi, Kazuhiro ; Niemann, Heiner. / Development to the blastocyst stage of parthenogenetically activated in vitro matured porcine oocytes after solid surface vitrification (SSV). In: Theriogenology. 2006 ; Vol. 66, No. 2. pp. 415-422.
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AU - Carnwath, Joseph W.

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