Development of Piezoelectric Immunosensor for the Detection of Probiotic Bacteria

Helga Szalontai, Nóra Adányi, A. Kiss

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A Quartz Crystal Microbalance (QCM) based direct immunosensor was developed for real-time detection of probiotic bacteria. To optimize the immunosensor system, model measurements were carried out with bovine serum albumin (BSA) and anti-BSA IgG (a-BSA) antibody. During the model experiments, two kinds of self-assembled monolayers were created to compare their efficiency on antigen binding. Sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido] hexanoate (sulfo-LC-SPDP) and 16-mercapto-hexadecanoic acid (MHDA) cross-linking agents were used for immobilizing anti-BSA antibody onto the gold surface of the AT-cut quartz wafer. Two different measuring procedures, flow-through and stopped-flow methods, were applied, and the frequency responses obtained by both analytical methods were compared.After the model experiments probiotic bacteria, Bifidobacterium bifidum O1356 and Lactobacillus acidophilus O1132 serotypes were detected from buffer solution and from real samples (spiked milk samples, acidophilus, and bifidus milk samples).Using the novel immunosensor, the target bacteria could be detected in the range of 104-107 colony-forming units (CFU)/ml within 60 minutes. The selectivity of a-Bifidobacterium bifidum and a-Lactobacillus acidophilus antibody coated sensors were also tested.

Original languageEnglish
Pages (from-to)1214-1229
Number of pages16
JournalAnalytical Letters
Volume45
Issue number10
DOIs
Publication statusPublished - Jul 1 2012

Keywords

  • Immunosensor
  • Piezoelectric biosensor
  • Probiotic bacteria
  • QcM
  • Quartz crystal microbalance

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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