Development of PCR-based markers on chromosome 5H for assisted selection of frost-tolerant genotypes in barley

B. Tóth, E. Francia, F. Rizza, A. M. Stanca, G. Galiba, N. Pecchioni

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Frost tolerance is an important trait for barley breeding. Field selection for this trait is not always efficient since, especially in Southern Europe, severe winter frost occurs erratically. Recent advances of cloned genes and molecular markers in barley provide molecular breeders with the means to develop new, simple PCR-based molecular markers, which can be used to select frost-tolerant genotypes quickly without stress simulation. This paper reports the development of two STS markers derived from the RFLP probes WG644 and PSR637, chosen as they are located on the long arm of homoeologous group 5 chromosomes of Triticeae, known to harbour the most important loci for frost tolerance. The two STS markers were validated together with one selected RAPD marker, OPA17, by separating two sets of winter and spring barley genotypes with different levels of frost tolerance. The ability of the developed markers to select segregant frost-tolerant and frost-susceptible genotypes was then investigated in a population of doubled haploid lines derived from a cross between a highly tolerant ('Nure') and a susceptible ('Tremois') genotype. In this population only two markers, OPA17 and Psr637 demonstrated their efficiency in dividing the phenotypes according to the parental alleles. These two markers mapped on the long arm of chromosome 5H, tightly linked to two frost tolerance QTLs. Two polymorphic bands of the WG644 STS were mapped: the former on the long arm of chromosome 5H (Wg644c) and the latter (Wg644b) on the long arm of chromosome 2H.

Original languageEnglish
Pages (from-to)265-273
Number of pages9
JournalMolecular Breeding
Volume14
Issue number3
DOIs
Publication statusPublished - Oct 2004

Fingerprint

frost resistance
Hordeum
Chromosomes
frost
Genetic Markers
barley
Genotype
chromosomes
Polymerase Chain Reaction
genotype
Chromosomes, Human, Pair 5
winter barley
genetic markers
Southern European region
Haploidy
spring barley
doubled haploids
Ports and harbors
Restriction Fragment Length Polymorphisms
Population

Keywords

  • Barley
  • Chromosome 5H
  • Frost tolerance; MAS
  • Molecular markers

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Biotechnology
  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Development of PCR-based markers on chromosome 5H for assisted selection of frost-tolerant genotypes in barley. / Tóth, B.; Francia, E.; Rizza, F.; Stanca, A. M.; Galiba, G.; Pecchioni, N.

In: Molecular Breeding, Vol. 14, No. 3, 10.2004, p. 265-273.

Research output: Contribution to journalArticle

Tóth, B. ; Francia, E. ; Rizza, F. ; Stanca, A. M. ; Galiba, G. ; Pecchioni, N. / Development of PCR-based markers on chromosome 5H for assisted selection of frost-tolerant genotypes in barley. In: Molecular Breeding. 2004 ; Vol. 14, No. 3. pp. 265-273.
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