Development of off-line layer chromatographic and total reflection X-ray fluorescence spectrometric methods for arsenic speciation

Victor G. Mihucz, Ágnes M. Móricz, Krisztina Kröpfl, Szilvia Szikora, Eniko Tatár, Lué Merú Marcó Parra, Gyula Záray

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Rapid and low cost off-line thin layer chromatography-total reflection X-ray fluorescence spectrometry and overpressured thin layer chromatography-total reflection X-ray fluorescence spectrometry methods have been developed for separation of 25 ng of each As(III), As(V), monomethyl arsonic acid and dimethylarsinic acid applying a PEI cellulose stationary phase on plastic sheets and a mixture of acetone/acetic acid/water = 2:1:1 (v/v/v) as eluent system. The type of eluent systems, the amounts (25-1000 ng) of As species applied to PEI cellulose plates, injection volume, development distance, and flow rate (in case of overpressured thin layer chromatography) were taken into consideration for the development of the chromatographic separation. Moreover, a microdigestion method employing nitric acid for the As spots containing PEI cellulose scratched from the developed plates divided into segments was developed for the subsequent total reflection X-ray fluorescence spectrometry analysis. The method was applied for analysis of root extracts of cucumber plants grown in As(III) containing modified Hoagland nutrient solution. Both As(III) and As(V) were detected by applying the proposed thin layer chromatography/overpressured thin layer chromatography-total reflection X-ray fluorescence spectrometry methods.

Original languageEnglish
Pages (from-to)1124-1128
Number of pages5
JournalSpectrochimica Acta - Part B Atomic Spectroscopy
Volume61
Issue number10-11 SPEC. ISS.
DOIs
Publication statusPublished - Nov 1 2006

Keywords

  • Arsenic
  • OPLC
  • Plant
  • Speciation
  • TLC
  • TXRF

ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Spectroscopy

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