Development of neurons containing acetylcholinesterase and cholinacetyltransferase in dispersed cell culture of rat cerebellum

P. Kása, K. Bánsághi

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Abstract

Cells from one-day-old cerebellum were grown for up to 30 days in dispersed cell culture. The characteristic neurons (deep cerebellar, Golgi and Purkinje cells) maintained their properties. It was found histochemically that some of the large cells display strong AChE activities in the perikaryon and in some processes, while biochemically the specific activities of the marker enzymes of the acetylcholine system, AChE (EC 3.1.1.7) and ChAc (EC 2.3.1.6), were increased and unchanged, respectively. During cultivation, the number of AChE-positive neurons increased. It can be inferred from these studies that, besides the AChE-positive (cholinoceptive) cells, ChAc-active (cholinergic) neurons (possibly Golgi II. type cells and some neurons in the deep cerebellar nuclei) are present in the cerebellum of the rat.

Original languageEnglish
Pages (from-to)263-270
Number of pages8
JournalHistochemistry
Volume61
Issue number3
DOIs
Publication statusPublished - Jan 1979

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Acetylcholinesterase
Cerebellum
Cell Culture Techniques
Neurons
Cerebellar Nuclei
Cholinergic Neurons
Choline O-Acetyltransferase
Purkinje Cells
Acetylcholine
Enzymes

ASJC Scopus subject areas

  • Anatomy

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Development of neurons containing acetylcholinesterase and cholinacetyltransferase in dispersed cell culture of rat cerebellum. / Kása, P.; Bánsághi, K.

In: Histochemistry, Vol. 61, No. 3, 01.1979, p. 263-270.

Research output: Contribution to journalArticle

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