Development of nano-hydroxyapatite graft with silk fibroin scaffold as a new bone substitute

Hae Yong Kweon, Kwang Gill Lee, Chang Hoon Chae, Csaba Balázsi, Soo Kee Min, Jwa Young Kim, Je Yong Choi, Seong Gon Kim

Research output: Contribution to journalArticle

39 Citations (Scopus)


Purpose: This study involves a comparison between the bone regeneration of nano-hydroxyapatite (nHA), as derived from eggshells either with or without silk fibroin scaffolds, and the unfilled control in the rabbit calvarial bony defect model. Materials and Methods: Sixteen 4-month-old New Zealand white rabbits, with a mean weight of 2.8 kg (range, 2.5-3.0 kg), were used in this experiment. After the formation of bilateral parietal bony defects (diameter, 8.0 mm), either an nHA or an nHA+silk fibroin combination (nHA+silk) was grafted. The control was unfilled defect. The bone regeneration was evaluated by micro-computed tomography (μCT) and histomorphometric analyses at 4 and 8 weeks. Results: All measured variables of the μCT analysis were significantly higher in the grafted groups (nHA and nHA+silk) than in the unfilled control groups at both 4 and 8 weeks after operation (P < .05). On histomorphometric analysis, there was no significant difference between the groups at 4 weeks after operation. However, the nHA group exerted significantly higher bone regeneration (40.16% ± 8.27%) compared with the unfilled control group (25.66% ± 10.98%) or the nHA+silk group (16.62% ± 3.05%) (P < .05). Conclusion: The nHA from eggshells exerted better bone formation than the unfilled control group on both μCT and histomorphometric analyses. Considering the rapid healing in bony defect and easy availability, the nHA from the eggshells could prove to be a good new bone substitute.

Original languageEnglish
Pages (from-to)1578-1586
Number of pages9
JournalJournal of Oral and Maxillofacial Surgery
Issue number6
Publication statusPublished - Jun 1 2011

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology

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