Development of female and male gametophytes in cereal species

I. Timár, Z. Pónya, L. Szabó, B. Barnabás

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The first steps in studies on the female and male gametophytes of wheat involved the light microscope analysis of semi-thin sections of embryo sacs containing egg-cells developing in planta. The information thus obtained on the development of the egg-cell from its initial formation to maturity contributed to the successful isolation of egg-cells. The morphological and ultrastructural details of egg-cells isolated 3, 6, 9, 12, 15 and 18 days after emasculation were examined to determine the function of the female gametophyte and its suitability for micromanipulation. A sufficient number of gametoplasts in the right stage of development are required for the successful microinjection and in vitro fusion of egg-cells and male gametes. No data are yet available on the fusiogenicity of wheat egg-cells in various stages of development. Various in vitro fertilisation and microinjection techniques could be of service in gamete fusion experiments aimed at the creation of interspecific and intergeneric hybrids which do not occur in nature due to sporophytic incompatibility. The results acquired in investigations on immature embryos can be used for the study of embryos developing from egg-cells fertilised in vitro.

Original languageEnglish
Pages (from-to)321-335
Number of pages15
JournalActa Agronomica Hungarica
Volume50
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

gametophytes
ova
embryo (plant)
germ cells
wheat
embryo sac
in vitro fertilization
light microscopes
Plantae
immatures

Keywords

  • Early embryo development
  • Egg-cell development
  • Morphology of isolated egg-cells
  • Morphology of young embryos
  • Pollen development

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Development of female and male gametophytes in cereal species. / Timár, I.; Pónya, Z.; Szabó, L.; Barnabás, B.

In: Acta Agronomica Hungarica, Vol. 50, No. 3, 2002, p. 321-335.

Research output: Contribution to journalArticle

Timár, I. ; Pónya, Z. ; Szabó, L. ; Barnabás, B. / Development of female and male gametophytes in cereal species. In: Acta Agronomica Hungarica. 2002 ; Vol. 50, No. 3. pp. 321-335.
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