Development of eight-plex microsatellite PCR for parentage control in deer

A. Zsolnai, I. Lehoczky, A. Gyurmán, J. Nagy, L. Sugár, I. Anton, P. Horn, I. Magyary

Research output: Contribution to journalArticle

Abstract

Nine loci have been compiled into two multiplex microsatellite polymerase chain reaction (PCR) sets (four and five loci) and used as a tool to determine the most probable hind for each calf. The two sets were suitable to combine them in an eight-plex reaction. The exclusion probabilities of the eight-plex reaction and the nine loci were 99.3 and 99.6 % respectively, which allows the routine application of eight loci in wildlife management- A s a first attempt to use molecular genetic information for such a task and it eliminates multiple sample handling in consecutive PCRs. Two loci out of the nine were never been used in deer previously.

Original languageEnglish
Pages (from-to)143-149
Number of pages7
JournalArchives Animal Breeding
Volume52
Issue number2
DOIs
Publication statusPublished - Oct 10 2009

Keywords

  • deer
  • microsatellite
  • parentage

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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