Nine loci have been compiled into two multiplex microsatellite polymerase chain reaction (PCR) sets (four and five loci) and used as a tool to determine the most probable hind for each calf. The two sets were suitable to combine them in an eight-plex reaction. The exclusion probabilities of the eight-plex reaction and the nine loci were 99.3 and 99.6% respectively, which allows the routine application of eight loci in wildlife management - as a first attempt to use molecular genetic information for such a task and it eliminates multiple sample handling in consecutive PCRs.Two loci out of the nine were never been used in deer previously.
|Number of pages||7|
|Journal||Archiv fur Tierzucht|
|Publication status||Published - Jun 25 2009|
ASJC Scopus subject areas
- Food Animals
- Animal Science and Zoology