Development of eight-plex microsatellite PCR for parentage control in deer

Attila Zsolnai, István Lehoczky, Alice Gyurmán, Janos Nagy, László Sugár, Istvan Anton, Peter Horn, István Magyary

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Nine loci have been compiled into two multiplex microsatellite polymerase chain reaction (PCR) sets (four and five loci) and used as a tool to determine the most probable hind for each calf. The two sets were suitable to combine them in an eight-plex reaction. The exclusion probabilities of the eight-plex reaction and the nine loci were 99.3 and 99.6% respectively, which allows the routine application of eight loci in wildlife management - as a first attempt to use molecular genetic information for such a task and it eliminates multiple sample handling in consecutive PCRs.Two loci out of the nine were never been used in deer previously.

Original languageEnglish
Pages (from-to)143-149
Number of pages7
JournalArchiv fur Tierzucht
Volume52
Issue number2
Publication statusPublished - Jun 25 2009

    Fingerprint

Keywords

  • Deer
  • Microsatellite
  • Parentage

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology

Cite this

Zsolnai, A., Lehoczky, I., Gyurmán, A., Nagy, J., Sugár, L., Anton, I., Horn, P., & Magyary, I. (2009). Development of eight-plex microsatellite PCR for parentage control in deer. Archiv fur Tierzucht, 52(2), 143-149.