Development of competitive mRNA PCR for the quantification of interleukin-6-responsive junB oncogene expression

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14 Citations (Scopus)

Abstract

The transcription factor junB belongs to the jun family of protooncogenes. The appearence of junB mRNA in hepatic cells is an extremely early and sensitive marker for the action of proinflammatory cytokines including interleukin-6. In this study, a competitive reverse transcription (RT)-PCR assay has been developed that is suitable for the quantitative determination of junB mRNA expression. This nonisotopic assay compared to other methods (e.g., Northern blot) is a fast and convenient way to determine the expression of the junB gene and thus the immediate concentration- and time-dependent action of interleukin-6. Because inteleukin-6 and interleukin- 6-type cytokines play a highly important regulatory role in various pathophysiologically important processes, such as hepatic acute-phase reaction, the quantitative assay of junB mRNA completes the scale of laboratory approaches in inflammation and among other pathological conditions.

Original languageEnglish
Pages (from-to)854-860
Number of pages7
JournalBioTechniques
Volume24
Issue number5
Publication statusPublished - 1998

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Oncogenes
Interleukin-6
Assays
Polymerase Chain Reaction
Messenger RNA
Cytokines
Acute-Phase Reaction
Transcription
Northern Blotting
Reverse Transcription
Hepatocytes
Transcription Factors
Genes
Inflammation
Gene Expression
Liver

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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abstract = "The transcription factor junB belongs to the jun family of protooncogenes. The appearence of junB mRNA in hepatic cells is an extremely early and sensitive marker for the action of proinflammatory cytokines including interleukin-6. In this study, a competitive reverse transcription (RT)-PCR assay has been developed that is suitable for the quantitative determination of junB mRNA expression. This nonisotopic assay compared to other methods (e.g., Northern blot) is a fast and convenient way to determine the expression of the junB gene and thus the immediate concentration- and time-dependent action of interleukin-6. Because inteleukin-6 and interleukin- 6-type cytokines play a highly important regulatory role in various pathophysiologically important processes, such as hepatic acute-phase reaction, the quantitative assay of junB mRNA completes the scale of laboratory approaches in inflammation and among other pathological conditions.",
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T1 - Development of competitive mRNA PCR for the quantification of interleukin-6-responsive junB oncogene expression

AU - Igaz, P.

AU - Fejér, G.

AU - Szalai, C.

AU - Tóth, S.

AU - Falus, A.

PY - 1998

Y1 - 1998

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AB - The transcription factor junB belongs to the jun family of protooncogenes. The appearence of junB mRNA in hepatic cells is an extremely early and sensitive marker for the action of proinflammatory cytokines including interleukin-6. In this study, a competitive reverse transcription (RT)-PCR assay has been developed that is suitable for the quantitative determination of junB mRNA expression. This nonisotopic assay compared to other methods (e.g., Northern blot) is a fast and convenient way to determine the expression of the junB gene and thus the immediate concentration- and time-dependent action of interleukin-6. Because inteleukin-6 and interleukin- 6-type cytokines play a highly important regulatory role in various pathophysiologically important processes, such as hepatic acute-phase reaction, the quantitative assay of junB mRNA completes the scale of laboratory approaches in inflammation and among other pathological conditions.

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