Development of bronchus-associated lymphoid tissue hyperplasia following lipopolysaccharide-induced lung inflammation in rats

Andrea Bánfi, L. Tiszlavicz, Edgár Székely, F. Peták, Valéria Tóth-Szüki, Levente Baráti, F. Bari, Zoltán Novak

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Gram-negative bacterial endotoxin lipopolysaccharide (LPS) administration has been used as an animal model of sepsis-related acute lung injury and adult respiratory distress syndrome (ALI/ARDS). This paper describes the lung histology following lung injury induced by the intraperitoneal (i.p.) administration of endotoxin to rats, in comparison with earlier findings. ALI was induced by the i.p. administration of Esherichia coli LPS 2 (n = 8) or 3 (n = 5) mg/kg, whereas physiological saline was administered to the control animals (n = 5). Eighteen hours after the LPS injections, the animals were euthanized. The lungs and heart were removed in one block for histological study (hematoxylin and eosin [HE], periodic acid-Schiff [PAS], Mason's trichrome; light microscopy). The lung tissue injury (bronchial wall, vessels, alveoli, interstitium) was graded via a scoring system (0 to 3+). The control animals showed intact lung tissue. Ten of the 13 LPS group had bronchus-associated lymphoid tissue (BALT) hyperplasia. Pathological signs of ALI/ARDS, diffuse alveolar damage (DAD) and emphysema, were observed in 5 and 8 cases, respectively. LPS injection induces primarily BALT hyperplasia and also the less characteristic DAD. This rat model is suitable for the investigation not only of ALI/ARDS but also of BALT hyperplasia occurring as a consequence of chronic pulmonary inflammatory processes.

Original languageEnglish
Pages (from-to)186-197
Number of pages12
JournalExperimental Lung Research
Volume35
Issue number3
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Lymphoid Tissue
Bronchi
Hyperplasia
Lipopolysaccharides
Rats
Pneumonia
Tissue
Acute Lung Injury
Adult Respiratory Distress Syndrome
Animals
Lung
Lung Injury
Endotoxins
Injections
Periodic Acid
Histology
Emphysema
Hematoxylin
Eosine Yellowish-(YS)
Optical microscopy

Keywords

  • ALI/ARDS
  • BALT hyperplasia
  • Histology
  • LPS
  • Rat

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Development of bronchus-associated lymphoid tissue hyperplasia following lipopolysaccharide-induced lung inflammation in rats. / Bánfi, Andrea; Tiszlavicz, L.; Székely, Edgár; Peták, F.; Tóth-Szüki, Valéria; Baráti, Levente; Bari, F.; Novak, Zoltán.

In: Experimental Lung Research, Vol. 35, No. 3, 04.2009, p. 186-197.

Research output: Contribution to journalArticle

Bánfi, Andrea ; Tiszlavicz, L. ; Székely, Edgár ; Peták, F. ; Tóth-Szüki, Valéria ; Baráti, Levente ; Bari, F. ; Novak, Zoltán. / Development of bronchus-associated lymphoid tissue hyperplasia following lipopolysaccharide-induced lung inflammation in rats. In: Experimental Lung Research. 2009 ; Vol. 35, No. 3. pp. 186-197.
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