Development of an enzyme-linked immunosorbent assay (ELISA) for the herbicide trifluralin

Gyöngyvér Hegedus, Iván Bélai, András Székács

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A sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the herbicide trifluralin. Polyclonal antibodies were developed in rabbits against a haptenic analogue N-(2,6-dinitro-4-trifluoromethylphenyl)-N-n-propyl-6-aminohexanoic acid. Using antibodies against this hapten, a hapten-heterologous competitive ELISA in the antigen-immobilized format allowed detection of the target analyte at concentrations between 0.1 and 100ngml-1. Closely related herbicides benfluralin, ethalfluralin cross-reacted with the antibodies in the optimized immunoassay at 5.2 and 2.9%, respectively, while less related dinitroaniline herbicides, e.g. isopropalin and pendimethalin did not show significant cross-reactivity. Various factors, e.g. the effect of pH and organic solvents have also been studied. The ELISA system tolerated methanol as a co-solvent at up to 5% content, while detection sensitivity appeared to be more vulnerable to other solvents including acetone. The optimized ELISA was found to be applicable to the detection of trifluralin in various matrices including surface water samples and vegetable juices. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)121-133
Number of pages13
JournalAnalytica Chimica Acta
Issue number2
Publication statusPublished - Sep 29 2000



  • Assay optimization
  • Detection in water and vegetable juices
  • GC-MS
  • Hapten synthesis
  • Immunoassay
  • SPME
  • Trifluralin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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