The objective of our project was to develop an analytical method which can measure the VOCs (volatile organic compounds) to find the important microbial metabolites to predict the spoilage during storage of fruits. As a model system plums (Prunus salicina) inoculated with Penicillium expansum were chosen. Analyses were performed by solid-phase microextraction (SPME) coupled to gas chromatography (GC) - mass spectrometry (MS). The current study employs multivariate experimental design for the optimization of 4 factors, namely extraction time, desorption time, agitation and desorption temperature, by a 24-1 experimental design. Desorption time and agitation showed no significant effect on the amount of extracted concentration. For extraction time 25 min. and 250 °C for desorption temperature were chosen for the 2 important factors. Four kinds of samples (agar inoculated with P. expansum, plum inoculated with P. expansum, control plum and control agar) were measured for 4 days. Styrene, 1-methoxy-3-methylbenzene, methyl linoleate and a 'non-identified aromatic compound', were reported here as possible P. expansum marker compounds.
- Gas chromatography-mass spectrometry (GC-MS)
- Penicillium expansum
- Solid phase microextraction (SPME)
- Volatile organic compounds (VOC)
ASJC Scopus subject areas
- Food Science