Development of a small-animal focal brain irradiation model to study radiation injury and radiation-injury modifiers

K. Hideghéty, Imola Plangár, Imola Mán, Gábor Fekete, Zoltán Nagy, Gábor Volford, Tünde Tkés, Emilia Szabó, Zoltán Szabó, Kitti Brinyiczki, Petra Mózes, István Németh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose: Our aim was to establish an effective small-animal focal brain radiation model for research on brain injuries. Material and methods: Groups of up to six rats were exposed to a range of doses from 120-40 Gy, at 10 intervals of a 6 MeV electron beam. Open-field motor functions and water maze learning-memory tests were performed after the irradiation at two-week intervals. Morphological changes were detected through repeated magnetic resonance imaging (MRI) monthly and were compared with the histopathological findings to determine if they predicted late microscopic changes. Results: The development of necrosis proved to be dose-dependent. 120 Gy resulted in serious deterioration within 4 weeks in all rats. Localized necrosis in one hemisphere was detected 2 months after the irradiation with ≥ 70 Gy, and 3 months after 40-60 Gy consistent for all animals. The Morris water maze (MWM) tests proved to be the most sensitive tool for the early detection of a brain functional impairment. MRI screening provided useful information on the development of radiation necrosis, which defined the time point for histological examinations. Conclusions: The described method permits accurate dose delivery to a definite part in one hemisphere of the brain for six rats at a time. Following complex examinations, a dose of 40 Gy and a follow-up time of 4 months are proposed for investigations on neuroradiation modifiers.

Original languageEnglish
Pages (from-to)645-655
Number of pages11
JournalInternational Journal of Radiation Biology
Volume89
Issue number8
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Radiation Injuries
Necrosis
Brain
Magnetic Resonance Imaging
Maze Learning
Radiation
Water
Brain Injuries
Electrons
Research

Keywords

  • Brain
  • Focal irradiation
  • Rat

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Development of a small-animal focal brain irradiation model to study radiation injury and radiation-injury modifiers. / Hideghéty, K.; Plangár, Imola; Mán, Imola; Fekete, Gábor; Nagy, Zoltán; Volford, Gábor; Tkés, Tünde; Szabó, Emilia; Szabó, Zoltán; Brinyiczki, Kitti; Mózes, Petra; Németh, István.

In: International Journal of Radiation Biology, Vol. 89, No. 8, 08.2013, p. 645-655.

Research output: Contribution to journalArticle

Hideghéty, K, Plangár, I, Mán, I, Fekete, G, Nagy, Z, Volford, G, Tkés, T, Szabó, E, Szabó, Z, Brinyiczki, K, Mózes, P & Németh, I 2013, 'Development of a small-animal focal brain irradiation model to study radiation injury and radiation-injury modifiers', International Journal of Radiation Biology, vol. 89, no. 8, pp. 645-655. https://doi.org/10.3109/09553002.2013.784424
Hideghéty, K. ; Plangár, Imola ; Mán, Imola ; Fekete, Gábor ; Nagy, Zoltán ; Volford, Gábor ; Tkés, Tünde ; Szabó, Emilia ; Szabó, Zoltán ; Brinyiczki, Kitti ; Mózes, Petra ; Németh, István. / Development of a small-animal focal brain irradiation model to study radiation injury and radiation-injury modifiers. In: International Journal of Radiation Biology. 2013 ; Vol. 89, No. 8. pp. 645-655.
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