Development of a sensitive ELISA for the determination of fumonisin B1 in cereals

I. Barna-Vetró, Erzsébet Szabó, B. Fazekas, L. Solti

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Monoclonal fumonisin B1 antibodies with high titer were raised by using FB1 - glutaraldehydekeyhole limpet hemocyanin immunogen prepared by a short cross-linker reagent (glutaraldehyde). Mean cross-reactivities of the selected monoclonal antibody for FB1, FB2, and FB3 were 100, 91.8, and 209%, respectively; no reactivity was found with hydrolyzed fumonisin. A direct, competitive enzyme-linked immunosorbent assay for the quantitative determination of FB1 in cereals has been developed with this antibody. Fifty percent acetonitrile-based solvent with some additives was used for extraction of cereals, and the diluted extracts were used without cleanup in the test. The mean within-assay and interassay coefficients of variation for the standard curve were 1. The toxin recovery from cereals infected with 50 - 200 ng/g of FB1 varied between 61 and 84%. According to the comparable results of naturally infected maize samples, this test proved to be suitable for the rapid screening of food and feed samples for the presence of FBs.

Original languageEnglish
Pages (from-to)2821-2825
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume48
Issue number7
DOIs
Publication statusPublished - Jul 2000

Fingerprint

fumonisin B1
Assays
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Fumonisins
Hemocyanin
Immunosorbents
Antibodies
Glutaral
Screening
antibodies
fumonisins
glutaraldehyde
Monoclonal Antibodies
cross reaction
Recovery
Zea mays
monoclonal antibodies
toxins
Enzymes

Keywords

  • Cereals
  • ELISA
  • FB-KLH
  • Fumonisin B

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Development of a sensitive ELISA for the determination of fumonisin B1 in cereals. / Barna-Vetró, I.; Szabó, Erzsébet; Fazekas, B.; Solti, L.

In: Journal of Agricultural and Food Chemistry, Vol. 48, No. 7, 07.2000, p. 2821-2825.

Research output: Contribution to journalArticle

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