Development of a sensitive ELISA for the determination of fumonisin B1 in cereals

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Abstract

Monoclonal fumonisin B1 antibodies with high titer were raised by using FB1 - glutaraldehydekeyhole limpet hemocyanin immunogen prepared by a short cross-linker reagent (glutaraldehyde). Mean cross-reactivities of the selected monoclonal antibody for FB1, FB2, and FB3 were 100, 91.8, and 209%, respectively; no reactivity was found with hydrolyzed fumonisin. A direct, competitive enzyme-linked immunosorbent assay for the quantitative determination of FB1 in cereals has been developed with this antibody. Fifty percent acetonitrile-based solvent with some additives was used for extraction of cereals, and the diluted extracts were used without cleanup in the test. The mean within-assay and interassay coefficients of variation for the standard curve were <10%. The measuring range of this test is 10 - 500 ng/g, with a detection limit of 7.6 ng/g FB1. The toxin recovery from cereals infected with 50 - 200 ng/g of FB1 varied between 61 and 84%. According to the comparable results of naturally infected maize samples, this test proved to be suitable for the rapid screening of food and feed samples for the presence of FBs.

Original languageEnglish
Pages (from-to)2821-2825
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume48
Issue number7
DOIs
Publication statusPublished - Jul 1 2000

Keywords

  • Cereals
  • ELISA
  • FB-KLH
  • Fumonisin B

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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