Development of a novel biosensor for the detection of arsenic in drinking water

J. Aleksic, F. Bizzari, Y. Cai, B. Davidson, K. De Mora, S. Ivakhno, S. L. Seshasayee, J. Nicholson, J. Wilson, A. Elfick, C. French, L. Kozma-Bognár, H. Ma, A. Millar

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We sought to develop a whole-cell biosensor for the detection of arsenic in drinking water, a major problem in Bangladesh and West Bengal. In contrast to previously described systems, our biosensor would give a pH change as output, allowing simple detection with a pH electrode or pH indicator solution. We designed and modelled a system based on the arsenate-responsive promoter of the Escherichia coli arsenic detoxification system, using urease to increase pH in the absence of arsenate, and β-galactosidase (LacZ) to decrease pH in the presence of arsenate. The pH-reducing β-galactosidase part of the system was constructed and tested, and was found to give a clear response to arsenate concentrations as low as 5ppb arsenic, well below the World Health Organisation (WHO) recommended limit of 10ppb.

Original languageEnglish
Pages (from-to)87-90
Number of pages4
JournalIET Synthetic Biology
Volume1
Issue number1-2
DOIs
Publication statusPublished - 2007

Fingerprint

Arsenic
Biosensing Techniques
Biosensors
Potable water
Drinking Water
Galactosidases
Detoxification
Escherichia coli
Urease
Health
Electrodes
Bangladesh
arsenic acid

ASJC Scopus subject areas

  • Molecular Biology
  • Biotechnology
  • Cell Biology
  • Bioengineering

Cite this

Aleksic, J., Bizzari, F., Cai, Y., Davidson, B., De Mora, K., Ivakhno, S., ... Millar, A. (2007). Development of a novel biosensor for the detection of arsenic in drinking water. IET Synthetic Biology, 1(1-2), 87-90. https://doi.org/10.1049/iet-stb:20060002

Development of a novel biosensor for the detection of arsenic in drinking water. / Aleksic, J.; Bizzari, F.; Cai, Y.; Davidson, B.; De Mora, K.; Ivakhno, S.; Seshasayee, S. L.; Nicholson, J.; Wilson, J.; Elfick, A.; French, C.; Kozma-Bognár, L.; Ma, H.; Millar, A.

In: IET Synthetic Biology, Vol. 1, No. 1-2, 2007, p. 87-90.

Research output: Contribution to journalArticle

Aleksic, J, Bizzari, F, Cai, Y, Davidson, B, De Mora, K, Ivakhno, S, Seshasayee, SL, Nicholson, J, Wilson, J, Elfick, A, French, C, Kozma-Bognár, L, Ma, H & Millar, A 2007, 'Development of a novel biosensor for the detection of arsenic in drinking water', IET Synthetic Biology, vol. 1, no. 1-2, pp. 87-90. https://doi.org/10.1049/iet-stb:20060002
Aleksic, J. ; Bizzari, F. ; Cai, Y. ; Davidson, B. ; De Mora, K. ; Ivakhno, S. ; Seshasayee, S. L. ; Nicholson, J. ; Wilson, J. ; Elfick, A. ; French, C. ; Kozma-Bognár, L. ; Ma, H. ; Millar, A. / Development of a novel biosensor for the detection of arsenic in drinking water. In: IET Synthetic Biology. 2007 ; Vol. 1, No. 1-2. pp. 87-90.
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