Development of a microtiter plate fluorescent assay for inhibition studies on the HTLV-1 and HIV-1 proteinases

P. Bagossi, János Kádas, Gabriella Miklóssy, Péter Boross, Irene T. Weber, J. Tőzsér

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The proteinase of human T-cell leukemia virus type-1 (HTLV-1), similar to the proteinase of human immunodeficiency virus type-1 (HIV-1), is a potential target for chemotherapy, since the virus is associated with a number of human diseases. A microtiter plate fluorescent assay was developed for the HTLV-1 and HIV-1 proteinases for direct comparison of the inhibition profiles of the enzymes. It was established that, except for Indinavir, none of the inhibitors designed against the HIV-1 proteinase were able to inhibit the HTLV-1 proteinase in the studied concentration range, while two reduced peptide bond-containing peptides having the sequence of HTLV-1 cleavage sites were inhibitors of the HTLV-1 proteinase. One of these was potent enough to be used for active site titration of the HTLV-1 proteinase.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalJournal of Virological Methods
Volume119
Issue number2
DOIs
Publication statusPublished - Aug 2004

Fingerprint

Deltaretrovirus
HIV-1
Peptide Hydrolases
Indinavir
Peptides
Catalytic Domain
Viruses
Drug Therapy
Enzymes

Keywords

  • CA
  • capsid protein
  • dimethyl sulfoxide
  • dithiothreitol
  • DMSO
  • DTT
  • HIV-1
  • HPLC
  • HTLV-1
  • human immunodeficiency virus type-1
  • human T-cell leukemia virus type-1
  • MA
  • matrix protein
  • NC
  • nucleocapsid protein
  • PR
  • proteinase
  • TFA
  • trifluoroacetic acid

ASJC Scopus subject areas

  • Virology

Cite this

Development of a microtiter plate fluorescent assay for inhibition studies on the HTLV-1 and HIV-1 proteinases. / Bagossi, P.; Kádas, János; Miklóssy, Gabriella; Boross, Péter; Weber, Irene T.; Tőzsér, J.

In: Journal of Virological Methods, Vol. 119, No. 2, 08.2004, p. 87-93.

Research output: Contribution to journalArticle

Bagossi, P. ; Kádas, János ; Miklóssy, Gabriella ; Boross, Péter ; Weber, Irene T. ; Tőzsér, J. / Development of a microtiter plate fluorescent assay for inhibition studies on the HTLV-1 and HIV-1 proteinases. In: Journal of Virological Methods. 2004 ; Vol. 119, No. 2. pp. 87-93.
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