Módszerfejlesztés szeleno-aminosavak és szelén(IV) közvetlen hidridfejlesztéssel történo meghatározásához

Translated title of the contribution: Development of a method for the determination of selenoamino acids and SeIV by direct hydride generation

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The speciation of selenocystine (SeCys), elenomethionine (SeMet), selenoethionine (SeEt) and Se(IV) was performed by on-line coupling of high performance liquid chromatography (HPLC) and atomic fluorescence spectrometry (AFS) with hydride generation (HG) as high efficiency sample introduction system. Reversed-phase liquid chromatography was applied to separate the selenium species with 10 mM ammonium-acetate buffer eluent adjusted to pH 4. The operating conditions for chromatographic separation (gradient elution, run time) and hydride generation (concentration of HCl and NaBH4 solution, argon and hydrogen flow rates) were carefully optimized. The efficiency of hydride generation for all of the four species was determined. Detection limits (DLs) obtained for SeCys, SeMet, SeEt and Se(IV) are 18, 70, 96, and 16 μg dm-3 respectively. The analytical performance of the method was controlled by measuring the selenium content of spiked selenium food supplements. The recoveries were in the range 90-110%.

Original languageHungarian
Pages (from-to)31-35
Number of pages5
JournalMagyar Kemiai Folyoirat, Kemiai Kozlemenyek
Volume107
Issue number1
Publication statusPublished - 2001

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Selenium
Hydrides
Acids
Argon
Liquid chromatography
High performance liquid chromatography
Spectrometry
Hydrogen
Buffers
Fluorescence
Flow rate
Recovery
selenocystine

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

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title = "M{\'o}dszerfejleszt{\'e}s szeleno-aminosavak {\'e}s szel{\'e}n(IV) k{\"o}zvetlen hidridfejleszt{\'e}ssel t{\"o}rt{\'e}no meghat{\'a}roz{\'a}s{\'a}hoz",
abstract = "The speciation of selenocystine (SeCys), elenomethionine (SeMet), selenoethionine (SeEt) and Se(IV) was performed by on-line coupling of high performance liquid chromatography (HPLC) and atomic fluorescence spectrometry (AFS) with hydride generation (HG) as high efficiency sample introduction system. Reversed-phase liquid chromatography was applied to separate the selenium species with 10 mM ammonium-acetate buffer eluent adjusted to pH 4. The operating conditions for chromatographic separation (gradient elution, run time) and hydride generation (concentration of HCl and NaBH4 solution, argon and hydrogen flow rates) were carefully optimized. The efficiency of hydride generation for all of the four species was determined. Detection limits (DLs) obtained for SeCys, SeMet, SeEt and Se(IV) are 18, 70, 96, and 16 μg dm-3 respectively. The analytical performance of the method was controlled by measuring the selenium content of spiked selenium food supplements. The recoveries were in the range 90-110{\%}.",
author = "I. Ipolyi and Z. Stef{\'a}nka and P. Fodor",
year = "2001",
language = "Hungarian",
volume = "107",
pages = "31--35",
journal = "Magyar Kemiai Folyoirat, Kemiai Kozlemenyek",
issn = "1418-9933",
publisher = "Magyar Kemikusok Egyesulete/Hungarian Chemical Society",
number = "1",

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T1 - Módszerfejlesztés szeleno-aminosavak és szelén(IV) közvetlen hidridfejlesztéssel történo meghatározásához

AU - Ipolyi, I.

AU - Stefánka, Z.

AU - Fodor, P.

PY - 2001

Y1 - 2001

N2 - The speciation of selenocystine (SeCys), elenomethionine (SeMet), selenoethionine (SeEt) and Se(IV) was performed by on-line coupling of high performance liquid chromatography (HPLC) and atomic fluorescence spectrometry (AFS) with hydride generation (HG) as high efficiency sample introduction system. Reversed-phase liquid chromatography was applied to separate the selenium species with 10 mM ammonium-acetate buffer eluent adjusted to pH 4. The operating conditions for chromatographic separation (gradient elution, run time) and hydride generation (concentration of HCl and NaBH4 solution, argon and hydrogen flow rates) were carefully optimized. The efficiency of hydride generation for all of the four species was determined. Detection limits (DLs) obtained for SeCys, SeMet, SeEt and Se(IV) are 18, 70, 96, and 16 μg dm-3 respectively. The analytical performance of the method was controlled by measuring the selenium content of spiked selenium food supplements. The recoveries were in the range 90-110%.

AB - The speciation of selenocystine (SeCys), elenomethionine (SeMet), selenoethionine (SeEt) and Se(IV) was performed by on-line coupling of high performance liquid chromatography (HPLC) and atomic fluorescence spectrometry (AFS) with hydride generation (HG) as high efficiency sample introduction system. Reversed-phase liquid chromatography was applied to separate the selenium species with 10 mM ammonium-acetate buffer eluent adjusted to pH 4. The operating conditions for chromatographic separation (gradient elution, run time) and hydride generation (concentration of HCl and NaBH4 solution, argon and hydrogen flow rates) were carefully optimized. The efficiency of hydride generation for all of the four species was determined. Detection limits (DLs) obtained for SeCys, SeMet, SeEt and Se(IV) are 18, 70, 96, and 16 μg dm-3 respectively. The analytical performance of the method was controlled by measuring the selenium content of spiked selenium food supplements. The recoveries were in the range 90-110%.

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