Development and identification of a 4HL.5DL wheat/barley centric fusion using GISH, FISH and SSR markers

K. Kruppa, E. Türkösi, É Szakács, A. Cseh, M. Molnár-Láng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The 4H(4D) wheat/barley substitution line was crossed with the 'Chinese Spring' ph1b mutant genotype in order to induce wheat-barley homoeologous recombinations. F3 and F4 seeds of the 4H(4D) × 'Chinese Spring' ph1b mutant cross were analysed using genomic in situ hybridization, and a Robertsonian translocation was detected in monosomic form. Disomic centric fusions were selected among the self-fertilized progenies. The presence of the long arm of 4H was confirmed with SSR markers. The long arm of the 5D wheat chromosome in the Robertsonian translocation was identified using fluorescent in situ hybridization with the help of three DNA probes: pSc119.2, Afa family and pTa71. The wheat/barley centric fusion was identified as a 4HL.5DL translocation. This line exhibited supernumerary spikelet character, but the number of seeds/plant did not increase. The 4HL.5DL centric fusion line is suitable genetic material to study the expression of genes located on 4HL in a wheat genetic background.

Original languageEnglish
Pages (from-to)221-229
Number of pages9
JournalCereal Research Communications
Volume41
Issue number2
DOIs
Publication statusPublished - Jun 1 2013

Fingerprint

Hordeum
Triticum
barley
wheat
Seeds
monosomics
disomics
mutants
substitution lines
DNA probes
DNA Probes
Spermatophytina
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
in situ hybridization
genetic background
Genetic Recombination
In Situ Hybridization
inflorescences
Chromosomes

Keywords

  • 'Chinese Spring' ph1b mutant
  • 4H(4D) substitution line
  • 4HL.5DL centric fusion
  • Triticum aestivum, Hordeum vulgare

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Physiology

Cite this

Development and identification of a 4HL.5DL wheat/barley centric fusion using GISH, FISH and SSR markers. / Kruppa, K.; Türkösi, E.; Szakács, É; Cseh, A.; Molnár-Láng, M.

In: Cereal Research Communications, Vol. 41, No. 2, 01.06.2013, p. 221-229.

Research output: Contribution to journalArticle

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