Development and evaluation of a perfusion decellularization porcine heart model: Generation of 3-dimensional myocardial neoscaffolds

Alexander Weymann, Sivakkanan Loganathan, Hiroaki Takahashi, Carsten Schies, Benjamin Claus, Kristóf Hirschberg, Pál Soós, Sevil Korkmaz, Bastian Schmack, Matthias Karck, Gábor Szabó

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Abstract

Background: Reports about the generation of 3-dimensional neoscaffolds for myocardial tissue engineering are limited. The architecture provided by perfusion decellularization of whole hearts would support the production of human-sized 3-dimensional living tissues from an acellular matrix. The aim of this study was to evaluate the potential of a perfusion decellularization model for whole heart tissue engineering. Methods and Results: Hearts were obtained from 12 German Landrace pigs from a selected abattoir. After preparation, the hearts were mounted and perfused on a modified Langendorff decellularization model specifically constructed for this reason. Decellularization was achieved by an ionic detergent-based perfusion protocol. The quality of the decellularization process was quantified by histology and fluorescence microscopy. Data regarding the presence of residual DNA within the decellularized hearts was measured with spectrophotometric quantification and compared to controls. After histological examination, all hearts lacked intracellular components but retained various types of collagen, proteoglycan and elastin. Quantitative DNA analysis demonstrated a significant reduction of DNA in decellularized hearts compared to controls (84.32±3.99 ng DNA/mg tissue vs. 470.13± 18.77 ng DNA/mg tissue (P<0.05)). Conclusions: The modified Langendorff perfusion decellularization model described here is applicable for whole porcine hearts by removing cellular content and DNA. The resulting 3-dimensional matrix provides an interesting tool for further studies in the field of whole heart tissue engineering.

Original languageEnglish
Pages (from-to)852-860
Number of pages9
JournalCirculation Journal
Volume75
Issue number4
DOIs
Publication statusPublished - Apr 1 2011

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Keywords

  • Cardiac tissue engineering
  • Decellularization
  • Organ perfusion

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Weymann, A., Loganathan, S., Takahashi, H., Schies, C., Claus, B., Hirschberg, K., Soós, P., Korkmaz, S., Schmack, B., Karck, M., & Szabó, G. (2011). Development and evaluation of a perfusion decellularization porcine heart model: Generation of 3-dimensional myocardial neoscaffolds. Circulation Journal, 75(4), 852-860. https://doi.org/10.1253/circj.CJ-10-0717